mRNA 3' end processing and metabolism / edited by Bin Tian.

Format:

Book

Contributor:

Tian, Bin, Ph.D., editor.

Series:

Methods in enzymology ; 0076-6879 v. 655.

Methods in enzymology, 0076-6879 ; volume 655

Language:

English

Subjects (All):

Messenger RNA.

Messenger RNA--Metabolism.

Genetic regulation.

Physical Description:

xx, 482 pages : illustrations (chiefly color) ; 24 cm.

Edition:

First edition.

Place of Publication:

Cambridge, MA : Academic Press, an imprint of Elsevier, 2021.

Contents:

Application and design considerations for 3'-end sequencing using click-chemistry / Madeline K. Jensen, Nathan D. Elrod, Hari Krishna Yalamanchili, Ping Ji, Ai Lin, Zhandong Liu, and Eric J. Wagner

PAS-seq 2 : A fast and sensitive method for global profiling of polyadenylated RNAs / Yoseop Yoon, Lindsey V. Soles, and Yongsheng Shi

TRENDseq : A highly multiplexed high throughput RNA 3' end sequencing for mapping alternative polyadenylation / Anton Ogorodnikov and Sven Danckwardt

QPAT-seq, a rapid and deduplicatable method for quantification of poly(A) site usages / Juncheng Lin, Congting Ye, and Qingshun Q. Li

Using TIF-Seq2 to investigate association between 5 ́ and 3 ́mRNA ends / Bingnan Li, Sueli Marques, Jingwen Wang, and Vicent Pelechano

Single-molecule polyadenylated tail sequencing (SM-PAT-Seq) to measure polyA tail lengths transcriptome-wide / Steven L. Coon, Tianwei Li, James R. Iben, Sandy Mattijssen, and Richard J. Maraia

3' end sequencing of pA⁺ and pA⁻ RNAs / Guifen Wu, Manfred Schmid, and Torben Heick Jensen

Comprehensive profiling of mRNA polyadenylation in specific cell types in vivo by cTag-PAPERCLIP / R. Samuel Herron and Hun-Way Hwang

A computational pipeline to infer alternative poly-adenylation from 3' sequencing data / Hari Krishna Yalamanchili, Nathan D. Elrod, Madeline K. Jensen, Ping Ji, Ai Lin, Eric J. Wagner, and Zhandong Liu

Systematic refinement of gene annotations by parsing mRNA 3' end sequencing datasets / Pooja Bhat, Thomas R. Burkard, Veronika A. Herzog, Andrea Pauli, and Stefan L. Ameres

Computational analysis of alternative polyadenylation from standard RNA-seq and single-cell RNA-seq data / Yipeng Gao and Wei Li

Quantifying alternative polyadenylation in RNAseq data with LABRAT / Austin E. Gillen, Raeann Goering, and J. Matthew Taliaferro

Poly(A) tail dynamics : Measuring polyadenylation, deadenylation and poly(A) tail length / Michael Robert Murphy, Ahmet Doymaz, and Frida Esther Kleiman

Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery / Yadong Sun, Wei Shen Aik, Xiao-Cui Yang, William F. Marzluff, Zbigniew Dominski, and Liang Tong

Comprehensive RNP profiling in cells identifies U1 snRNP complexes with cleavage and polyadenylation factors active in telescripting / Zhiqiang Cai, Byung Ran So, and Gideon Dreyfuss

Simultaneous studies of gene expression and alternative polyadenylation in primary human immune cells / Joana Wilton, Michael Tellier, Takayuki Nojima, Angela M. Costa, Maria Jose Oliveira, and Alexandra Moreira

RIPiT-Seq : A tandem immunoprecipitation approach to reveal global binding landscape of multisubunit ribonucleoproteins / Zhongxia Yi and Guramrit Singh

Generation of 3'UTR knockout cell lines by CRISPR/Cas9-mediated genome editing / Sibylle Mitschka, Mervin M. Fansler, and Christine Mayr

Modulation of alternative cleavage and polyadenylation events by dCas9-mediated CRISPRpas / Jihae Shin, Ruijia Wang, and Bin Tian.

Notes:

Includes bibliographical references.

Other Format:

ebook version :

ISBN:

9780128235737

012823573X

OCLC:

1231956157