Cyanobacterial lifestyle and its applications in biotechnology edited by Prashant Singh, Maria Fillat and Ajay Kumar

Format:

Book

Contributor:

Singh, Prashant, editor.

Fillat, Maria, editor.

Kumar, Ajay, PhD, editor.

Language:

English

Subjects (All):

Cyanobacteria--Biotechnology.

Cyanobacteria.

Physical Description:

1 online resource

Place of Publication:

London Academic Press [2022]

Contents:

Front cover

Half title

Full title

Copyright

Contents

Contributors

About the editors

Preface

1

Cyanobacterial diversity concerning the extreme environment and their bioprospecting

1.1 Introduction

1.2 Systematic study of extremophiles cyanobacteria

1.2.1 Thermophiles

1.2.2 Psychrophiles

1.2.3 Halophiles

1.2.4 Acidophiles

1.2.5 Alkaliphiles

1.2.6 Xerophilic

1.3 Application of extremophile cyanobacteria

1.4 Conclusions

References

Chapter 2

Cyanobacterial nanoparticles: Application in agriculture and allied sectors

2.1 Introduction

2.2 Nanobiotechnology

2.3 Nanoparticles

2.3.1 Types of NPs

2.4 Cyanobacterial NPs

2.5 Synthesis of NPs

2.5.1 Physical synthesis

2.5.2 Chemical synthesis

2.5.3 Biological synthesis of NPs

2.5.3.1 Intracellular green synthesis of NPs

2.5.3.2 Extracellular green synthesis of NPs

2.5.4 Cyanobacteria as a source of NPs synthesis

2.5.4.1 Cyanobacterial way of intracellular synthesis of NPs

2.5.4.2 Cyanobacterial way of extracellular synthesis of NPs

2.5.4.2.1 Cell-free media

2.5.4.2.2 Cell biomass filtrate

2.5.4.2.3 Biomolecule-based NP synthesis

2.6 Characterization of NPs

2.7 Mode of action

2.8 Applications of cyanobacterial-based NPs

2.9 Future projections of cyanobacteria-based NPs

2.10 Limitations of NPs

2.11 Conclusion

Chapter 3

Cyanobacterial photosynthetic reaction center in wobbly light: Modulation of light energy by orange carotenoid ...

3.1 Introduction

3.2 Fates of light energy absorbed by pigments

3.3 Light-harvesting complex organization in cyanobacteria

3.4 Modulation of light energy in cyanobacteria (photoprotective mechanism)

3.4.1 Photoprotective mechanism mediated through phycobilisome

3.4.2 The orange carotenoid proteins (OCP)

3.4.3 Full antenna capacity recovery: the role of FRP

3.5 Mechanism of OCP-mediated light modulation in cyanobacteria

3.5.1 Cyanobacterial reaction center in high light: OCP is a significant game-changer

3.5.2 Cyanobacterial reaction center under low light/dark: FRP is a significant game-changer

3.6 Conclusions

4

Back to the past: Improving photosynthesis with cyanobacterial genes

4.1 Introduction

4.2 Engineering cyanobacterial genes not related to photosynthesis

4.2.1 Stress response

4.2.2 Amino acid metabolism

4.2.3 Lipid metabolism

4.3 Manipulation of cyanobacterial genes related to photosynthesis in plants

4.3.1 Introduction of CCMs in plants

4.3.2 Manipulation of carbon assimilation

4.3.3 Manipulation of carbon uptake/transport

4.3.4 Sugar partitioning and utilization

4.3.5 Introduction of cyanobacterial proteins into the photosynthetic electron transport chain

4.3.5.1 The cyanobacterial lost genes: flavodoxin as a source for photosynthesis stress protection

4.3.5.2 The cyanobacterial lost genes: flavo-diiron proteins, boosting higher plants photosynthetic processes

4.4 Concluding remarks

5

Promises and challenges for expanding the use of N 2 -fixing cyanobacteria as a fertilizer for sustainable agriculture

5.1 Food security, sustainable agriculture, and N-fertilizers

5.2 Biological nitrogen fixation

5.3 Cyanobacterial BNF

5.3.1 Evolutionary origins

5.3.2 Ecological implications of N 2 -fixing cyanobacteria

5.3.3 Nitrogenase enzyme

5.3.3.1 nif genes

5.3.3.2 Nitrogenase sensitivity to oxygen

5.3.4 N 2 fixation in HC: spatial separation

5.3.4.1 N 2 fixation in NHC: temporal separation only, or temporal plus spatial separation ( Trichodesmium)

5.4 Cyanobacteria as biofertilizers

5.4.1 Living cyanobacteria-dependent traits

5.4.1.1 Release of fixed N 2

5.4.1.2 Release of fixed carbon

5.4.1.3 Enhancement of phosphorus availability

5.4.1.4 Release of phytohormones

5.4.2 Cyanobacterial biomass-dependent traits: mineralization pathway

5.5 Cyanobacteria and microalgae mass culture technology

5.5.1 Open systems

5.5.1.1 Raceway ponds

5.5.1.2 Thin-layer cascades

5.5.1.3 Turf scrubbers

5.5.2 Closed systems

5.6 Use of wastewater for cyanobacteria culture

5.7 Downstream process: harvesting and drying processes

5.8 Large-scale project for cyanobacterial- or microalgal biomass-based fertilizers

Acknowledgments

Chapter 6

Thermophilic and thermotolerant cyanobacteria: Environmental and biotechnological perspectives

6.1 Introduction

6.2 Thermophilic cyanobacteria diversity

6.3 Temperature stress responses in thermophiles cyanobacteria

6.4 Biotechnological application of thermophilic cyanobacteria

6.5 Metabolic engineering in cyanobacteria

6.6 Conclusion

Chapter 7

Exploring the ability of cyanobacterial ferric uptake regulator (FUR) proteins to increase yeast tolerance to ...

7.1 Introduction

7.2 Materials and methods

7.2.1 Strains and growth conditions

7.2.2 Cloning and transformation procedures

7.2.3 Western blot

7.2.4 Construction of the Green fluorescent protein (GFP)-tagged S. cerevisiae

7.2.5 Fluorescence microscopy

7.3 Results

7.3.1 Generation of S. cerevisiae strains expressing FurA and FurB from Anabaena sp. PCC 7120

7.3.2 Expression of FurB in S. cerevisiae increases its sensitivity to copper and manganese

7.3.3 The presence of FurB in S. cerevisiae enhances the effects of membrane-damaging compounds and saline stress

7.3.4 Fur proteins confer increased tolerance to oxidative stress in S. cerevisiae

7.3.5 Recombinant FurB is located in the cytosol of S. cerevisiae

7.4 Discussion

Chapter 8

Exploring ecological diversity and biosynthetic potential of cyanobacteria for biofuel production

8.1 The biosynthetic potential of cyanobacteria

8.2 Genomic diversity and genetic tools for cyanobacteria

8.3 Cyanobacterial biofuels

8.4 Hydrogen biofuel

Chapter 9

Cyanobacterial availability for CRISPR-based genome editing: Current and future challenges

9.1 Introduction

9.2 CRISPR/Cas9-based genome editing in cyanobacteria

9.3 CRISPR/Cas9-mediated cyanobacterial genome editing

9.4 CRISPR/Cas12a-mediated genome editing in cyanobacteria

9.5 Dead Cas9 (dCas9) and cyanobacterial gene expression

9.6 dCas9 offers an alternative approach for cyanobacterial metabolic engineering

9.7 Cyanobacterial genome editing offers markerless selection and gene multiplexing

9.8 Cyanobacterial genome editing: key challenges

9.9 Conclusion and prospects

Chapter 10

Cyanobacteria and salinity stress tolerance

10.1 Introduction

10.2 Distribution of cyanobacteria in the saline ecosystem

10.3 Sensing salinity by cyanobacterial cell

10.3.1 Salinity sensing by SOS pathway

10.4 Physiological and biochemical responses

10.4.1 Photosynthesis

10.4.2 Plasma membrane

10.4.3 Nitrogen fixation

10.4.4 Formation of ROS and antioxidative defense system

10.5 Accumulation of compatible solutes

10.5.1 Biosynthesis of compatible solutes

10.5.1.1. Glucosyl glycerate (GG) synthesis

10.5.2 Glycine betaine synthesis

10.5.3 Sucrose synthesis

10.5.4 Trehalose synthesis

10.6 Mechanism of salt tolerance

10.6.1 Stress response proteins

10.6.1.1 Na+ influx

10.6.1.2 Na + efflux

10.6.1.3 Limitation of K + uptake

10.7 Role of cyanobacteria in the remediation of salt-affected soil

10.8 Conclusion

Chapter 11

Cyanobacteria as biostimulants in the paddy fields

11.1 Introduction

11.2 Cyanobacterial biostimulants and plant growth-promoting potential

11.3 Cyanobacteria and their extracts: impacts on crops' productivity

11.3.1 Role of cyanobacterial metabolites in soil health improvement

11.3.1.1 Nitrogen fixation

11.3.1.2 Soils nutrients' bioavailability

11.3.1.3 Amendments in soil physical and chemical properties

11.3.3 Crops direct growth stimulation

11.3.4 Crops' protection against stresses

11.3.4.1 Protection against abiotic stresses

11.3.4.2 Protection against biotic stresses

11.4 Cyanobacteria as biostimulants in agriculture

11.5 Other biostimulants and their role in plant growth stimulations

11.6 Challenges involved in using cyanobacteria as biostimulants

11.7 Prospects and conclusion

Acknowledgment

Chapter 12

Molecular characterization of local cyanobacterial isolates using 16S rRNA , rpo B, and nif H biomarkers

12.1 Introduction

12.2 Molecular markers used to assess cyanobacterial biodiversity

12.2.1 16S rRNA gene

12.2.2 RNA polymerase

12.2.3 nif H gene

12.3 Biodiversity documentation

12.4 Molecular characterization of local cyanobacterial isolates

12.5 Phylogenetic analysis of local cyanobacterial isolates using three different biomarkers

12.5.1 16S rRNA gene-based phylogenetic tree

12.5.2 rpo B gene-based phylogenetic tree

12.5.3 nif H gene-based phylogenetic tree

Notes:

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Other Format:

Print version:

ISBN:

9780323910491

0323910491

OCLC:

1273676697

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