Essential techniques for medical and life scientists. A guide to contemporary methods and current applications with the protocols. Part 2 / edited by Prof. Yusuf Tutar.

Format:

Book

Author/Creator:

Tutar, Yusuf, Author.

Contributor:

Tutar, Yusuf, editor.

Language:

English

Subjects (All):

Medical sciences--Research.

Medical sciences.

Life sciences.

Spectrum analysis.

Physical Description:

1 online resource (166 pages)

Edition:

1st ed.

Place of Publication:

Singapore : Bentham Science Publishers Pte Ltd., [2020]

Summary:

This handbook covers some primary instruments-based techniques used in modern biological science and medical research programs. Key features of the book include introductory notes for each topic, a systematic presentation of relevant methods, and troubleshooting guides for practical settings. Topics covered in part 2 include: · Fourier transform mid-infrared (FT-MIR) spectroscopy · High performance liquid chromatography (HPLC) · Raman spectroscopy · Circular dichroism (CD) spectroscopy · Transmission electron microscopy (TEM) · Scanning electron microscopy (SEM) · SEM-EDX and its applications in plant science This book is a simple, useful handbook for students and teachers involved in graduate courses in life sciences and medicine. Readers will learn about the basics of featured techniques, the relevant applications and the established protocols.

Contents:

Cover

Title

Copyright

End User License Agreement

Contents

Foreword

Preface

List of Contributors

Fourier-Transform Mid-Infrared (FT-MIR) Spectroscopy in Biomedicine

Bernardo Ribeiro da Cunha1,2, Luís Ramalhete1,3, Luís P. Fonseca2 and Cecília R.C. Calado1,*

1. INTRODUCTION

2. DISPERSIVE VERSUS INTERFEROGRAM BASED EQUIPMENT

3. TYPES OF SAMPLES, DETECTION MODES AND DATA ANALYSIS METHODS

4. FT-MIR SPECTROSCOPY AND SYSTEMS BIOLOGY

5. NIR SPECTROSCOPY (NIRS)

6. RAMAN SPECTROSCOPY

7. DATA ACQUISITION MODES, PRE-PROCESSING AND PROCESSING METHODS

7.1. Spectra Acquisition Modes

7.2. Quality Tests And Spectral Pre-processing

7.3. Biochemical And Metabolic Interpretation

7.4. Dimensionality Reduction

7.5. Pattern Recognition And Multivariate Regression

7.6. Advanced Algorithms

8. APPLICATIONS IN CLINICAL DIAGNOSIS

8.1. Cancer

8.2. Brain Disorders

FINAL REMARKS

CONSENT FOR PUBLICATION

CONFLICT OF INTEREST

ACKNOWLEDGEMENTS

REFERENCES

High Performance Liquid Chromatography (HPLC) - Theoretical and Practical Aspects

Esen Tutar*

2. HPLC INSTRUMENT

2.1. Solvent Reservoir (Mobile Phase)

2.2. Pump (Solvent Delivery System)

2.2.1. Pump Types

2.2.2. Isocratic And Gradient Elution Mode

2.3. Sample Injection System

2.4. The Column (The stationary phase)

2.4.1. The Column Types

2.4.2. The Column Care, Maintenance And Storage

2.5. Detectors

2.5.1. Detector Characteristics

2.5.2. Detector Types

3. HPLC TYPES

4. HPLC PROTOCOLS

4.1. Sample Preparation

4.2. Quantitative Analysis

5. HPLC APPLICATIONS

Raman Spectroscopy and Its Biomedical Applications

Charu Arora1,*, Pathik Maji1 and P. K. Bajpai2

1. INTRODUCTION.

2. THEORETICAL BACKGROUND

2.1. Raman Scattering

2.2. Instrumentation for Raman Spectroscopy

2.3. Instrument Set Up

2.3.1. Source

2.3.2. Sample Handing Techniques

2.3.3. Filter

2.3.4. Monochromator

2.3.5. Detector

2.4. Scanning of Raman Spectra

3. TECHNIQUES OF RAMAN SPECTROSCOPY

3.1. Confocal Raman Spectroscopy

3.2. Raman Micro Spectroscopy

3.3. Selective-sampling Raman micro-spectroscopy

3.4. Surface Enhanced Raman Spectroscopy

3.5. Spatially Offset Raman Spectroscopy and Transmission Raman Spectroscopy

3.6. Fiber-Optic Raman Probes

3.7. Coherent Anti-Stokes Raman Spectroscopy and Stimulated Raman Spectroscopy

4. BIOMEDICAL APPLICATIONS

4.1. Raman Based Study Of Human Skin

4.2. Measurement Condition

4.3. In Vivo Cancer Detection by Raman Spectroscopy

4.4. Biofluid Diagnostic Assays Based on Raman Spectroscopy

4.5. Raman Analysis Of Sperm Nuclear DNA Integrity

4.6. Raman Investigation Of Microorganisms On Single Cell Level

4.7. Investigating the Atherosclerosis Process

4.8. Thrombosis Mechanisms Using High Resolution Surface Enhanced Raman Spectroscopy

4.9. Structure Determination Of Gelonin As A Biomolecule By Raman Spectroscopy

4.9.1. Classification

4.9.2. Measurement

4.9.3. Assignment of Proteins

CONCLUSION

Circular Dichroism

Yusuf Tutar1,2,*

2. INSTRUMENTATION AND SAMPLE PREPARATION

3. DATA COLLECTION AND PROCESSING

4. APPLICATIONS

4.1. Secondary Structure Content Of Proteins

4.1.1. Detecting Conformational Alterations Upon Ligand Binding

4.2. Protein Stability

5. PROTOCOL FOR CD MEASUREMENT

6. SUMMARY

REFERENCES.

Transmission Electron Microscopy: Theory and Applications

Charu Arora*, Sanju Soni and Pathik Maji

2. THEORY

3. INSTRUMENTATION

4. PREPARATION OF SAMPLES AND WORKING APPLICATIONS

5. CHALLENGES WITH BIOLOGICAL ELECTRON MICROSCOPY

5.1. Vacuum

5.2. Lack of Contrast

5.3. Transparency

5.4. Charging

6. DEVELOPMENT OF SCANNING TRANSMISSION ELECTRON MICROSCOPE

Scanning Electron Microscopy: Theory and Applications

Charu Arora*, Sumantra Bhattacharya, Sanju Soni and Pathik Maji

2. INSTRUMENTATION

3. SAMPLE PREPARATION

4. APPLICATIONS OF SCANNING ELECTRON MICROSCOPY (SEM)

SEM-EDX: A Potential Tool for Studies of Medicinal Plants

Iqbal Ansari1, Muniyan Sundararajan1, Ritesh Kumar1, Sadanand Sharma1 and Charu Arora2,*

2. DETAILS OF SEM-EDS

3. PRINCIPLE, WORKING AND PROCEDURE

4. LITERATURE REVIEW WITH EMPHASIS ON SEM-EDX STUDY OF MEDICINAL PLANTS

5. MEDICINAL PLANTS IN JHARKHAND STATE: AN OVERVIEW

6. SIGNIFICANCE

Subject Index

Back Cover.

Notes:

Description based on print version record.

ISBN:

9789811464867

9811464863

OCLC:

1235966457