Principles of Fluorescence Spectroscopy / edited by Joseph R. Lakowicz.

Format:

Book

Author/Creator:

Lakowicz, Joseph R.

Contributor:

Lakowicz, Joseph R., Editor.

Language:

English

Subjects (All):

Analytical chemistry.

Chemistry, Physical and theoretical.

Biophysics.

Analytical Chemistry.

Physical Chemistry.

Local Subjects:

Analytical Chemistry.

Physical Chemistry.

Biophysics.

Physical Description:

1 online resource (XXV, 698 p.)

Edition:

2nd ed. 1999.

Place of Publication:

New York, NY : Springer US : Imprint: Springer, 1999.

Language Note:

English

Summary:

`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface.

Contents:

1. Introduction to Fluorescence

2. Instrumentation for Fluorescence Spectroscopy

3. Fluorophores

4. Time-Domain Lifetime Measurements

5. Frequency-Domain Lifetime Measurements

6. Solvent Effects on Emission Spectra

7. Dynamics of Solvent and Spectral Relaxation

8. Quenching of Fluorescence

9. Advanced Topics in Fluorescence Quenching

10. Fluorescence Anisotropy

11. Time-Dependent Anisotropy Decays

12. Advanced Anisotropy Concepts

13. Energy Transfer

14. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers

15. Energy Transfer to Multiple Acceptors, in One, Two, or Three Dimensions

16. Protein Fluorescence

17. Time-Resolved Protein Fluorescence

18. Excited-State Reactions

19. Fluorescence Sensing

20. Long-Lifetime Metal-Ligand Complexes

21. DNA Technology

22. Phase-Sensitive and Phase-Resolved Emission Spectra

Appendix I. Corrected Emission Spectra

1. ?-Carboline Derivatives as Fluorescence Standards

2. Corrected Emission Spectra of 9,10-Diphenylanthracene, Quinine Sulfate, and Fluorescein

3. Long-Wavelength Standards

4. Ultraviolet Standards

5. Additional Corrected Emission Spectra

References

Appendix II. Fluorescent Lifetime Standards

1. Nanosecond Lifetime Standards

2. Picosecond Lifetime Standards

3. Representative Frequency-Domain Intensity Decays

4. Time-Domain Lifetime Standards

Appendix III. Additional Reading

1. Lifetime Measurements

2. Which Molecules are Fluorescent Representative Emission Spectra, and Practical Advice

3. Theory of Fluorescence and Photophysics

4. Principles of Fluorescence Spectroscopy

5. Biochemical Fluorescence

6. Protein Fluorescence

7. Data Analysis and Nonlinear Least Squares

8. Photochemistry

9. Flow Cytometry.-10. Phosphorescence

11. Polymer Science

12. Fluorescence Sensing

13. Immunoassays

14. Latest Applications of Fluorescence

15. Infrared and NIR Fluorescence

16. Lasers

17. Fluorescence Microscopy

18. Metal-Ligand Complexes and Unusual Luminophores

Answers to Problems.

Notes:

Includes index.

ISBN:

1-4757-3061-6