Ion channels : channel biochemistry, reconstitution, and function / edited by Daniel L. Minor, Jr., Henry M. Colecraft.

Format:

Book

Contributor:

Minor, Daniel L., editor.

Colecraft, Henry M., editor.

Series:

Issn

Language:

English

Subjects (All):

Ion channels.

Genre:

Laboratory manuals.

Physical Description:

1 online resource (358 pages)

Edition:

1st ed.

Place of Publication:

Cambridge, Massachusetts : Academic Press, [2021]

Summary:

Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic.

Contents:

Section I. Structure/function relationships. Correlating ion channel structure and function / Philipp A.M. Schmidpeter and Crina M. Nimigean ; TRPV3 expression and purification for structure determination by Cryo-EM / Arthur Neuberger, Kirill D. Nadezhdin, and Alexander I. Sobolevsky ; Methods to study phosphoinositide regulation of ion channels / Yevgen Yudin, Luyu Liu, Janhavi Nagwekar, and Tibor Rohacs ; Recombinant expression and purification of pentameric ligand-gated ion channels for Cryo-EM structural studies / Arvind Kumar, Sandip Basak, and Sudha Chakrapani ; Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography / Andrea Saponaro, Atiyeh Sadat Sharifzadeh, and Anna Moroni

Section II. Structural and chemical methods to assess channel dynamics. Structural dynamics of channels and transporters by high-speed atomic force microscopy / George R. Heath, Yi-Chih Lin, Tina R. Matin, and Simon Scheuring ; Crosslinking glutamate receptor ion channels / Andrew J.R. Plested and Mette H. Poulsen ; Single molecule FRET methodology for investigating glutamate receptors / Nabina Paudyal, Nidhi Kaur Bhatia, and Vasanthi Jayaraman ; Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues / Megumi Yamashita and Murali Prakriya

Section III. Channel reconstitution and studies in bilayers. A quantitative flux assay for the study of reconstituted Cl⁻ channels and transporters / Eva Fortea and Alessio Accardi ; Ion channel reconstitution in lipid bilayers / Eleonora Zakharian ; Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers / Oliver Rauh, Kerri Kukovetz, Laura Winterstein, Bianca Introini, and Gerhard Thiel

Section IV. Methods to assess oligomerization. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture / Randy B. Stockbridge.

Notes:

Description based on print version record.

Description based on publisher supplied metadata and other sources.

ISBN:

0-323-85375-7

0-323-85374-9

OCLC:

1255233581