Laboratory practices in microbiology / Osman Erkmen.

Format:

Book

Author/Creator:

Erkmen, Osman, 1955-

Language:

English

Subjects (All):

Microbiology--Laboratory manuals.

Microbiology.

Genre:

Laboratory manuals.

Electronic books.

Physical Description:

1 online resource

Place of Publication:

London : Academic Press, 2021.

System Details:

text file

Contents:

Section I Microbiological media and cultural techniques p. 1

1 Preparation of media and sterilization techniques p. 3

1.1 Theoretical information p. 3

1.2 Techniques in the preparation of media p. 12

2 Microorganisms in environment p. 19

2.1 Theoretical information p. 19

2.2 Microbial detection techniques from environment p. 19

3 Aseptic techniques p. 25

3.1 Theoretical information p. 25

3.2 Types of disinfectants and antiseptics p. 29

3.3 Aseptic transfer techniques in microbiological researches p. 31

4 Pure culture techniques p. 41

4.1 Theoretical information p. 41

4.2 Plate culture techniques p. 42

5 Culture preservation techniques p. 51

5.1 Theoretical information p. 51

5.2 Preservation techniques p. 52

Section II Techniques on morphological examination of bacteria p. 59

6 Microscope for study of microorganisms p. 61

6.1 Light microscope p. 61

6.2 Uses of microscope p. 67

7 Microbial cell measurement technique p. 71

7.1 Theoretical information p. 71

7.2 Cell measurement technique p. 75

8 Wet-mount and hanging-drop techniques p. 83

8.1 Theoretical information p. 83

8.2 Microscopic techniques p. 84

9 Smear preparation and simple staining techniques p. 91

9.1 Theoretical information p. 91

9.2 Staining techniques p. 92

10 Gram staining technique p. 99

10.1 Theoretical information p. 99

10.2 Gram staining p. 101

11 Bacterial endospore staining techniques p. 107

11.1 Theoretical information p. 107

11.2 Endospore staining techniques p. 108

12 Bacterial capsule staining techniques p. 115

12.1 Theoretical information p. 115

12.2 Capsule staining techniques p. 116

13 Motility tests p. 123

13.1 Theoretical information p. 123

13.2 Motility indication techniques p. 124

Section III Biochemical and cultural characteristics of microorganisms p. 133

14 Cultural characteristics of bacteria p. 135

14.1 Theoretical information p. 135

14.2 Techniques used in the indication of cultural characteristics p. 135

15 Oxidation and fermentation p. 143

15.1 Theoretical information p. 143

15.2 Fermentation and oxidation tests p. 145

16 Hydrolytic reactions p. 155

16.1 Theoretical information p. 155

16.2 Hydrolytic reactions on different substrates p. 155

16.3 Hydrolytic reaction tests p. 157

Section IV Chemical and physical effects on microorganisms p. 163

17 Antiseptic and disinfectant effects on microorganisms p. 165

17.1 Theoretical information p. 165

17.2 Antiseptics and disinfectants antimicrobial sensitivity test techniques p. 166

18 Antibiotic sensitivity test technique p. 181

18.1 Theoretical information p. 181

18.2 Kirby-Bauer antibiotic sensitivity test technique p. 182

19 Effect of hand scrubbing on removal of microorganisms p. 187

19.1 Theoretical information p. 187

19.2 Hand scrubbing p. 187

20 Temperature effects on microorganisms p. 193

20.1 Theoretical information p. 193

20.2 Indication of temperature effects on microorganisms p. 194

21 Osmotic pressure and pH effects on microorganisms p. 199

21.1 Theoretical information p. 199

21.2 Effects of osmotic pressure and pH on microorganisms p. 201

22 Ultraviolet light effects on microorganisms p. 205

22.1 Theoretical information p. 205

22.2 Ultraviolet light application p. 206

23 Oxygen effects on microorganisms p. 211

23.1 Theoretical information p. 211

23.2 Determination of oxygen effects on microorganisms p. 212

Section V Fungi and bacteriophages p. 219

24 Morphological characteristics of yeasts and molds p. 221

24.1 Theoretical information p. 221

24.2 Morphological characteristics fungi p. 225

25 Isolation, enumeration, and uses of bacteriophages p. 231

25.1 Theoretical information p. 231

25.2 Uses of bacteriophages p. 232

25.3 Life cycle of bacteriophages p. 234

25.4 Bacteriophage culturing techniques p. 236.

Notes:

Includes bibliographical references and index.

ISBN:

9780323900157

0323900151

OCLC:

1237252931

Access Restriction:

Restricted for use by site license.