HLA from benchtop to bedside / Arthur Bradley Eisenbrey.

Format:

Book

Author/Creator:

Eisenbrey, A. Bradley.

Language:

English

Subjects (All):

HLA histocompatibility antigens.

Genre:

Electronic books.

Physical Description:

1 online resource

Place of Publication:

London : Academic Press, 2021.

System Details:

text file

Contents:

1 Introduction and some history p. 1

Fundamentals: innate and adaptive immunity p. 2

2 The structure of the major histocompatibility complex and major HLA components p. 5

HLA class I p. 8

Function of HLA Class la molecules p. 9

HLA lb molecules p. 13

Other HLA class I histocompatibility genes: MICA and MICB p. 13

HLA class II p. 14

3 Nomenclature and naming conventions for HLA p. 23

HLA allele naming p. 27

4 The crossmatch p. 33

Microcytotoxicity and the "NIH standard" technique p. 35

Antihuman globulin p. 38

Crossmatching by microcytotoxicity p. 39

ABO and solid organ transplantation p. 40

T and B cell isolation p. 40

Immunofluorescence p. 41

Flow cytometry p. 42

HLA antibody detection in tissues p. 47

5 HLA antibody detection (screening) and identification p. 55

Antibody identification by microlymphocytotoxicity p. 56

Interpretation of antibody specificity in cell-based assays p. 57

Panel reactive antibody p. 58

Solid-phase antibody screening and identification p. 59

Commercial solid-phase HLA antibody assays are qualitative, not quantitative p. 62

Clinical indications for HLA antibody testing p. 70

Single antigen bead result interpretation in clinical practice p. 73

MFI as a quantitative result p. 75

Virtual crossmatch p. 76

Management of antibody-mediated transplant rejection p. 78

HLA in Transfusion Medicine and Blood Banking p. 78

6 HLA typing by serologic methods p. 85

7 HLA typing by molecular methods p. 89

Molecular HLA typing "before PCR" p. 90

Oligonucleotide probing and endonuclease cutting p. 94

Polymerase chain reaction and the molecular revolution in clinical medicine p. 95

Polymerase chain reaction sequence specific primers p. 97

PCR-RFLP p. 99

PCR-rSSO p. 100

High resolution typing ambiguities in molecular HLA typing p. 102

The challenge of null alleles p. 107

rtPCR (aka qPCR, real-time PCR, and quantitative PCR) p. 108

8 Massively parallel (next generation) sequencing p. 111

NGS sequencing by Hybridization and Ligation p. 115

Selection of molecular HLA typing methods p. 116

9 HLA alleles, disease associations, and HLA pharmacogenetics p. 119

The mathematics of disease associations p. 120

How do HLA alleles cause disease? p. 123

10 Engraftment monitoring p. 129

A little history p. 130

Microsatelllte DNA, VNTRs, STRs p. 130

Engraftment monitoring p. 132

Engraftment monitoring by capillary electrophoresis p. 133

Alternative platforms for engraftment monitoring p. 136.

Notes:

Includes index.

ISBN:

9780128242377

012824237X

OCLC:

1229125509

Access Restriction:

Restricted for use by site license.