Enzyme engineering and evolution : specific enzyme applications / edited by Dan S. Tawfik.

Format:

Book

Contributor:

Tawfik, Dan S., editor.

Series:

Methods in enzymology ; v. 644.

Methods in enzymology ; volume 644

Language:

English

Subjects (All):

Enzymes.

Enzymology.

Enyzmes.

Medical Subjects:

Enyzmes.

Physical Description:

xvi, 296 pages : illustrations (some color) ; 24 cm.

Edition:

First edition.

Place of Publication:

Cambridge, MA : Academic Press, 2020.

Contents:

1. Engineering lytic polysaccharide monooxygenases (LPMOs) / Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink

2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases / Daniela M. Carminati, Eric J. Moore and Rudi Fasan

3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries / Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer

4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution / Duncan S. Macdonald and Donald Hilvert

5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling / Lukas Deweid, Adem Hadjabdelhafid-Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier

6. Engineering aldolases for asymmetric synthesis / Mikael Widersten

7. Ultra-high throughput screening for novel protease specificities / Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke

8. Directed evolution of transcriptional switches using dual-selector systems / Yuki Kimura and Daisuke Umeno

9. Methods for competitive enrichment and evaluation of superior DNA ligases / Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley

10. Directed evolution of custom polymerases using droplet microfluidics / Derek Vallejo, Ali Nikoomanzar and John C. Chaput

11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution / Pu Xue, Tong Si and Huimin Zhao

12. Split enzymes: Design principles and strategy / Shion A. Lim and James A. Wells.

Notes:

Includes bibliographical references.

Other Format:

ebook version :

ISBN:

9780128244319

0128244313

OCLC:

1163955502