Detection of pathogens in water using micro and nano-technology / [editors] Giampaolo Zuccheri and Nikolaos Asproulis.

Format:

Book

Author/Creator:

Zuccheri, Giampaolo, Editor.

Contributor:

Zuccheri, Giampaolo.

Asproulis, Nikolaos.

Language:

English

Subjects (All):

Water--Microbiology.

Water.

Pathogenic microorganisms--Detection.

Pathogenic microorganisms.

Nanotechnology.

Microtechnology.

Physical Description:

1 online resource (316 p.)

Place of Publication:

London ; New York : IWA Publishing, 2012.

Language Note:

English

Summary:

Detection of Pathogens in Water Using Micro and Nano-Technology aims to promote the uptake of innovative micro and nano-technological approaches towards the development of an integrated, cost-effective nano-biological sensor useful for security and environmental assays. The book describes the concerted efforts of a large European research project and the achievements of additional leading research groups. The reported knowledge and expertise should support in the innovation and integration of often separated unitary processes. Sampling, cell lysis and DNA/RNA extraction, DNA hybridisation detection micro- and nanosensors, microfluidics, together also with computational modelling and risk assessment can be integrated in the framework of the current and evolving European regulations and needs. The development and uptake of molecular methods is revolutionizing the field of waterborne pathogens detection, commonly performed with time-consuming cultural methods. The molecular detection methods are enabling the development of integrated instruments based on biosensor that will ultimately automate the full pathway of the microbiological analysis of water.

Contents:

Contents

Preface

Contributors

Chapter 1: Overview of European regulation and standards on microbiological water analysis / Sabine Müller and Jonathan Loeffler

1.1. Introduction

1.2. European Regulation on Microbiological Analysis of Drinking Water

1.3. European Regulation on Microbiological Analysis of Recreational Water

1.4. European and International Standards for Microbiological Water Analysis

Chapter 2: Risk analysis of bio-terroristic attacks on drinking water systems / Christian Mittermayr

2.1. Introduction

2.2. Definitions

2.3. Risk Analysis for Terrorism

2.4. Risk Analysis of Bio-Terroristic Attacks on Drinking Water Systems

2.5. Risk Estimation

Chapter 3: Sample collection procedures for Online Contaminant Monitoring System / Miloslava Proksˇova´, Marianna Cíchová and Lívia Tóthová

3.1. Introduction

3.2. Microbial Monitoring of Drinking Water

3.3. Sampling Plan

3.4. New Security Approaches for Drinking Water

3.5. New Approach of Online Contamination Monitoring Device (OCMD)

3.5.1. Location of OCMD in water system

Chapter 4: A device to extract highly diluted specimens out of large volumes of water for analysis in lab-on-a-chip detection systems / Christoph Zeis

4.1. The Need for a Macro-to-Micro-Fluidic Interface

4.2. Principles of Separation

4.3. The Needle in the Haystack

4.4. Technical Description of the DINAMICS Concentration Apparatus

4.5. Conclusions

Chapter 5: Sustainable DNA/RNA release methods for in-line waterborne pathogen screening devices / Hunor Sántha

5.1. Introduction

5.2. Survey on Pathogen-Lysis/Cell-Disruption Methods

5.3 Considerations for Water Samples and In-line (Quasi Continuous) operation

5.4 The Realised Case in The DINAMICS Project

Chapter 6: The microsystem based core of the DINAMICS water testing system: Design considerations and realization of the chip units / Theo T. Veenstra

6.1 Microfluidics in DINAMICS

6.2 Layout of the Fluidic Parts of DINAMICS

6.3 Microfluidic Components in DINAMICS

6.4 Current Status

6.5 Conclusion

Chapter 7: Electrochemical biosensor strategies for pathogen detection in water security / Daniele Gazzola, Simone Bonetti and Giampaolo Zuccheri

7.1 Introduction

7.2 Biosensor Strategies for Pathogen Detection in Water Security

7.3 Common Electrochemical Detection Systems for DNA Biosensors

7.4 The On-Chip Simplified Electrochemical Technique Developed in the DINAMICS Project

Chapter 8: Biochemical and Nanotechnological Strategies for Signal Enhancement in the Detection of Nucleic Acids with Biosensors / Alessandra Vinelli, Manuele Onofri and Giampaolo Zuccheri8.1 Introduction

8.2 Enhancement Methods Based on Enzymatic (or Catalyzed) Reactions

8.3 Enhancement Methods Based on Nanoparticles or Nanostructures

8.4 Method Based on DNA Nanostructures

8.5 Conclusions and Perspectives

Chapter 9: Computational modelling of aqueous environments in micro and nanochannels / D. Mantzalis, K. Karantonis, N. Asproulis, L. Könözsy and D. Drikakis

9.1 Introduction

9.2 Effects of Physical Characteristics

9.3 Computational Approaches

9.4 Liquid Flow in Confined Geometries

Chapter 10: Computational recipes of transport phenomena in micro and nanofluidics / N. Asproulis10.1 Introduction

10.2 Modelling Approaches

10.3 Meta-Modelling for Macromolecules

10.4 Hybrid Continuum-Molecular Models

References

Chapter 11: Multi-detection of waterborne pathogens in raw and treated water samples by using ultrafiltration concentration and DNA array technology / Sophie Courtois, Anne Cajon, Aurore Romey, Fanny Poyet and Claude Mabilat

11.1 Introduction

11.2 Improved and Simplified Method for Concentrating Viral, Bacterial and Protozoan Pathogens

11.3 Integrated Protocol for Nucleic Acid Extraction, Amplification and Sequence Identification Through High Density Microarray

11.4 Results

11.5 Conclusions

Chapter 12: Detection and enumeration of waterborne mycobacteria / Joseph O. Falkinham III

12.1 Ecology of Waterborne Mycobacteria

12.2 Physiological Ecology of Waterborne Mycobacteria

12.3 Risk Analysis and Source-Tracking Environmental Mycobacteria

12.4 Sampling and Sample Treatment Strategies for Mycobacterial Detection and Enumeration

12.5 Mycobacterial Detection or Enumeration

Chapter 13: New molecular technologies for the rapid detection of Legionella in water / E. Soria, M. A. Yáñez, R. Múrtula and V. Catalán

13.1 Introduction

13.2 Immunodetection and Legionella Fast Detection

13.3 Legionella Detection using Microfluidics

13.4 Future Research Directions

Chapter 14: Detection of virus in the water environment / Johan Nordgren and Lennart Svensson

14.1 Introduction

14.2 Concentration of Virus from Water Samples

14.3 Detection and Quantification Methods

14.4 Perspectives

Chapter 15: Design of PCR primers for the detection of waterborne bacteria / Julien Gardès and Richard Christen

15.1 Introduction

15.2 The Target Genes

15.3 Design of PCR Primers

15.4 DNA-Based Detection Technologies

15.5 Conclusions

Chapter 16: Fluid structure and boundary slippage in nanoscale liquid films / Nikolai V. Priezjev

16.1 Abstract

16.2 Introduction

16.3 Molecular Dynamics Simulation Model

16.4 Results

16.5 Conclusions

Chapter 17: Understanding slip at the nanoscale in fluid flows using atomistic simulations / T. E. Karakasidis and A. Liakopoulos

17.1 Introduction – Definition of Slip

17.2 Importance of Slip

17.3 Experimental Measurement of Slip

17.4 Atomistic Simulations

17.5 Atomistic Simulations Results About Slip

17.6 Conclusions

References.

Notes:

Description based upon print version of record.

Includes bibliographical references.

CC BY-NC-ND

ISBN:

9781780401096

1780401094

OCLC:

843212986

Access Restriction:

Open access Unrestricted online access