Polymerase chain reaction : theory and technology / Mark A. Behlke [and twenty eight others].

Format:

Book

Author/Creator:

Behlke, Mark A., author.

Language:

English

Subjects (All):

Polymerase chain reaction.

Physical Description:

1 online resource (269 pages)

Edition:

1st ed.

Place of Publication:

Norfolk, UK : Caister Academic Press, [2019]

Summary:

This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. Topics covered include: principles of PCR, fluorescent chemistries, instrumentation, quantification strategies, extraction and purification of nucleic acids, sample preparation, controls for validation, primers and probes, standardization of methods, MIQE guidelines, mRNA expression and PCR arrays. This book provides a comprehensive overview of PCR theory, instrumentation and methods. The book represents an excellent, detailed guide for anyone interested in the development and use of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.

Contents:

Intro

Contents

Chapter 1 Introduction to the Real-time Polymerase Chain Reaction

Chapter 2 Principles of the Real-time Polymerase Chain Reaction

Chapter 3 Homogenous Fluorescent Chemistries for Real-time PCR

Chapter 4 Instrumentation and Fluorescent Chemistries Used in Quantitative Polymerase

Chapter 5 Quantification Strategies in Real-time Polymerase Chain Reaction

Chapter 6 The Extraction and Purification of Nucleic Acids for Analysis by PCR

Chapter 7 Sample Preparation for Real-time PCR in Food Science

Chapter 8 Internal and Other Controls for Real-time PCR Validation

Chapter 9 Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase

Chapter 10 Internal Amplification Controls in Real-time Polymerase Chain Reaction-Based

Chapter 11 Standardization of Real-time PCR Methods in Food Microbiology

Chapter 12 MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay

Chapter 13 Analysis of mRNA Expression by Real-time PCR

Chapter 14 Real-time PCR Arrays.

Notes:

Description based on print version record.

ISBN:

1-912530-25-2