Enzyme activity in single cells / edited by Nancy L. Allbritton, Michelle L. Kovarik.

Format:

Book

Contributor:

Allbritton, Nancy L., editor.

Kovarik, Michelle L. (Michelle Lynn), editor.

Series:

Methods in enzymology ; 0076-6879 v. 628.

Methods in enzymology, 0076-6879 ; volume 628

Language:

English

Subjects (All):

Enzymes--Analysis.

Enzymes.

Cells--Measurement--Methodology.

Cells.

Cells--Analysis.

Cytology--Technique.

Cytology.

Cytology--Methodology.

Cytophotometry.

Cells--Spectroscopic imaging.

Cells--Microscopy.

Microfluidics.

Cell separation--Methodology.

Cell separation.

Cytology--Research--Methodology.

Cytology--Research.

Methodology.

Microscopy.

Spectroscopic imaging.

Cells--Measurement.

Physical Description:

xvii, 307 pages : illustrations (some color) ; 24 cm.

Edition:

First edition.

Place of Publication:

Cambridge, MA : Academic Press, an imprint of Elsevier, 2019.

Summary:

"Single-cell analysis is rapidly expanding area of research, and a growing number of analytes ranging from nucleic acid material to proteins to small molecules can now be characterized at the single-cell level. Interest in single-cell measurements has surged as the importance of cellular heterogeneity in both normal and pathological processes has become clear. While variation between cells arises at multiple points in cellular physiology, differences in enzymatic activity are a key driver of nongenetic cellular heterogeneity. Understanding the causes and effects of this heterogeneity is an important problem for basic science and clinical medicine. For example, cell-to-cell variation in enzyme activity has important implications for druggable targets, since variations in enzyme activity likely suggests variation in drug response between cells. As a result, a variety of methods have recently been developed to address the challenges of single-cell enzyme assays. Single-cell measurements are challenging particularly for mammalian or microbial cells since these entities are small, complex, dynamic systems, and each method presented in this volume addresses these challenges in unique ways. The first section of this volume presents techniques based on microscopy and spectroscopy. The second section details a variety of methods that involve physical containment of cells or cell contents in small volumes, using traditional microfluidics, droplet-based microfluidics, or other microscale structures to facilitate analysis. Finally, the third section describes mass spectrometric measurements of single cells with an emphasis on both instrumentation and chemical tools to assist these analyses ... This volume is a comprehensive overview of the leading techniques in single-cell enzyme measurements, including instrumentation, molecular tools, and integrated devices. We hope that the detailed outlines of cutting-edge procedures will make single-cell measurements of enzyme activity more accessible and more widely used, advancing both our biological understanding of cellular heterogeneity and leading to the development of even more advanced techniques and tools."--Preface

Contents:

Phase-separated condensates of metabolic complexes in living cells : purinosome and glucosome / Songon An, Miji Jeon, Erin L. Kennedy, and Minjoung Kyoung

Synthetic probe development for measuring single or few-cell activity and efflux / Alison Lui, Jeffrey Wang, Linda Chio, and Markita P. Landry

Enzymatic activity in single cells / Josephine Geertsen Keller, Magnus Stougaard, and Birgitta R. Knudsen

Single-cell assays using integrated continuous-flow microfluidics / Ee Xien Ng, Myat Noe Hsu, Guoyun Sun, and Chia-Hung Chen

Single-cell activity screening in microfluidic droplets / Stefanie Neun, Tomasz S. Kaminski, and Florian Hollfelder

The microfluidic capture of single breast cancer cells for multi-drug resistance assays / Karan Parekh, Hamide Sharifi, Avid Khamenehfar, Timothy V. Beischlag, Robert T.M. Payer, and Paul C.H. Li

Single-cell proteolytic activity measurement using microfluidics for rare cell populations / Yu-Chih Chen and Euisik Yoon

Microfluidic technology for investigation of protein function in single adherent cells / Aldo Jesorka, Inga Põldsalu, and Irep Gözen

Nanokits for the electrochemical quantification of enzyme activity in single living cells / Rongrong Pan and Dechen Jiang

Design of an automated capillary electrophoresis platform for single-cell analysis / David H. Abraham, Matthew M. Anttila, Luke A. Gallion, Brae V. Petersen, Angela Proctor, and Nancy L. Allbritton

Microelectrophoretic single-cell measurements with microfluidic devices / Jay Sibbitts, Jalal Sadeghi, and Christopher T. Culbertson

Chemical probes for spatially resolved measurement of active enzymes in single cells / Gang Li and Raymond E. Moellering

Single-cell proteomics in complex tissues using microprobe capillary electrophoresis mass spectrometry / Camille Lombard-Banek, Sam B. Choi, and Peter Nemes

Electroosmotic extraction coupled to mass spectrometry analysis of metabolites in live cells / Ruichuan Yin, Venkateshkumar Prabhakaran, and Julia Laskin.

Notes:

Includes bibliographical references.

Other Format:

ebook version :

ISBN:

9780128170908

0128170905

OCLC:

1096517155