Live cell optical microscopy with limited light dose / by Herbert Schneckenburger, Verena Richter, and Michael Wagner.

Format:

Book

Author/Creator:

Schneckenburger, Herbert, author.

Richter, Verena, author.

Wagner, Michael, author.

Contributor:

Society of Photo-Optical Instrumentation Engineers, publisher.

Series:

SPIE spotlight ; SL42.

SPIE digital library

SPIE. Spotlight ; SL42

Language:

English

Subjects (All):

Medical microscopy.

Microscopy.

Medical Subjects:

Microscopy.

Genre:

Electronic books.

Physical Description:

1 online resource (vi, 62 pages).

Place of Publication:

Bellingham, Washington : SPIE, 2018.

System Details:

Mode of access: World Wide Web.

text file

Summary:

Live-cell microscopy is a rapidly growing field for the detection of cell or tissue architecture and for cell metabolism. This Spotlight examines the early detection of diseases and cellular responses to pharmaceutical agents with 3-D systems and non-phototoxic light doses. Techniques discussed in this eBook include 3-D microscopy, super-resolution microscopy, membrane imaging, long-exposure imaging, and spectral imaging.

Contents:

1 Introduction: 1.1. Challenge 1: 3-D microscopy; 1.2. Challenge 2: super-resolution microscopy; 1.3. Challenge 3: membrane imaging; 1.4. Challenge 4: long time exposure; 1.5. Further challenges

2. Methods and applications: 2.1. Cell cultivation; 2.2. Wide-field microscopy; 2.3. Confocal laser scanning microscopy; 2.4. Light-sheet fluorescence microscopy; 2.5. Structured illumination microscopy; 2.6. Axial tomography; 2.7. Total internal reflection fluorescence microscopy; 2.8. Spectral imaging, FLIM, and FRET

3 Concluding remarks.

Notes:

"SPIE Digital Library."--Website.

Title from PDF title page (SPIE eBooks Website, viewed 2018-08-03).

ISBN:

9781510622586

OCLC:

1048293587

Access Restriction:

Restricted for use by site license.