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Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual / Ryan Mehl.
- Format:
- Book
- Author/Creator:
- Mehl, Ryan, author.
- van Zee, Kari, author.
- Kean, Kelsey, author.
- Series:
- Open textbook library.
- Language:
- English
- Subjects (All):
- Chemistry--Textbooks.
- Chemistry.
- Genre:
- Textbooks.
- Physical Description:
- 1 online resource.
- Distribution:
- Minneapolis, MN Open Textbook Library.
- Place of Publication:
- Corvallis, Oregon Oregon State University 2019.
- Language Note:
- In English.
- System Details:
- data file
- Summary:
- Chemical Biology and Biochemistry Laboratory Using Genetic Code Expansion Manual
- Contents:
- 1. Background
- 2. Overview of Laboratory Responsibilities Tentative Schedule
- 3. Objectives for Week 1
- 4. Concepts for Choosing and Expressing Mutants
- 5. Objectives for Week 2
- 6. Concepts for Protein Expression
- 7. Methods for Protein Expression
- 8. Concepts for SDS-PAGE
- 9. Methods for SDS-PAGE
- 10. Objectives for Week 3
- 11. Concepts for Protein Purification and Desalting
- 12. Methods for Protein Purification and Desalting
- 13. Microfluidizer Lysis Method
- 14. Objectives for Week 4
- 15. Methods for Protein Standard Curves and SDS-PAGE
- 16. Objectives for Weeks 5-9
- 17. Concepts for Enzyme Assays
- 18. Methods for Enzyme Assays
- Notes:
- Description based on print resource
- Access Restriction:
- Open Access Unrestricted online access
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