Applications of molecular microbiological methods / edited by Torben Lund Skovhus, Sean M. Caffrey, Casey R.J. Hubert.

Format:

Book

Contributor:

Skovhus, Torben Lund, editor.

Caffrey, Sean M., editor.

Hubert, Casey R. J., editor.

Language:

English

Subjects (All):

Molecular microbiology.

Molecular microbiology--Methodology.

Physical Description:

1 online resource (234 p.)

Edition:

1st ed.

Place of Publication:

Norfolk, England : Caister Academic Press, [2014]

Language Note:

English

Summary:

Innovative, constructive, and continually evolving technologies are propelling microbiology into an exciting new era. This new era will witness the harnessing and control of complex microbial communities in a huge variety of applications in the industrial, medical, and environmental spheres. State-of-the-art tools are being developed and utilized to analyze the molecules that microorganisms possess and generate, including DNA, RNA, proteins, lipids, and cellular metabolites. This book, written by international experts in the field, presents emerging molecular methods that allow the diversity o

Contents:

Contents; Contributors; Foreword ; Introduction; Overview of chapters; Part I: Industrial Microbiology; 1: Molecular Methods in Microbiologically Influenced Corrosion Research, Monitoring and Control; Introduction; PCR-based techniques; Hybridization techniques; Novel approaches; Conclusion and perspectives; 2: Using the Power of Molecular Microbiological Methods in Oilfield Corrosion Management; Background; Methods; Results; Conclusions and perspectives; 3: Next-generation Sequencing Approach to Understand Pipeline Biocorrosion; Introduction; Molecular methods; Investigation of MIC

Case study4: Molecular Microbiological Methods Applied on Ship Ballast Tank Samples; Introduction; Case; Experimental set-up; Results; Electrochemical data; Discussion; 5: Molecular Characterization of Microbial Communities Associated with ALWC; Introduction; Material and methods; Results and discussion; Conclusions towards a further understanding of ALWC; 6: Application of the qPCR Technique for SRB Quantification in the Oil and Gas Industries; Background; Methods; Results and discussion; Conclusions and perspectives

7: Molecular Microbiological Methods Applied to Microbial Methane ProductionIntroduction; Prospects for enhanced methanogenesis from oil reservoirs, coal beds and shale; Use of MMMs to elucidate microbial communities and activities in oil reservoirs, coal beds and shale; Case study 1: the use of MMM to pinpoint the key microbial players involved in biodegrading crude oil hydrocarbons in a high-temperature crude oil reservoir; Case study 2: the use of MMM to characterize the microbial diversity and methanogenic potential of coal seams in the Western Canadian Sedimentary Basin

Part II: Medical Microbiology8: Characterization of Bacterial Communities in Suspected Prosthetic Joint Infections; Introduction; Material and methods; Results; Discussion; Conclusion; 9: Using the Core and Supra Genomes to Determine Diversity and Natural Proclivities; Definition of species in post-genomic era; Genomic diversity within bacterial species; Sources of genomic variability: horizontal gene transfer and point mutations; The supragenome provides a framework for understanding intra-species bacterial diversity in the post-genomic era; Mathematical models to represent supragenomes

Environmental conditions that support HGTConclusion; Part III: Environmental Microbiology; 10: Quantitative PCR and Reverse Transcription Quantitative PCR Applied to Methane-cycling Archaea; Introduction; Methods; Results; Discussion; 11: The Metabolic Function of Uncultured Microorganisms; Introduction; Methods for studying single cells; Case study 1: discovery of novel carbon fixing microorganisms by genomic analyses of single cells; Untangling the genomic information from a single cell; Studying physiological capabilities in single microbial cells; Conclusion and outlook

12: Assessing Microbial Activity and Degradation Pathways in the Environment

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on print version record.

ISBN:

1-908230-69-X

OCLC:

874179228