Insect molecular viology : advances and emerging trends / edited by Bryony C. Bonning.

Format:

Book

Contributor:

Bonning, Bryony C., editor.

Language:

English

Subjects (All):

Insects--Viruses.

Insects.

Insects--Virus diseases.

Physical Description:

1 online resource (272 pages)

Edition:

1st ed.

Place of Publication:

Norfolk, UK : Caister Academic Press, [2019]

Summary:

The volume opens with a description of the insect virome and the explosion in discovery of new viral taxa. The following four chapters focus on anti-viral immunity including endogenous viral elements some of which may provide the molecular basis for long-term anti-viral immunity, the discovery of new viral suppressors of RNA interference, the role of new classes of small RNA molecules in dictating infection outcomes, and the Drosophila-dicistrovirus model as a powerful resource for insect molecular virology. The application of omics tools to insect-vectored plant viral disease, recent advances in tetravirus, polydnavirus, and baculovirus research are then described. The final chapters review progress in baculovirus expression vector and surface display technologies for use in laboratory and therapeutic applications. Written by leading experts, this work is essential reading for students and scholars of insect virology and immunology and provides a valuable resource for users of baculovirus-derived tools.

Contents:

Intro

Contents

Current Books of Interest

Preface

1: The Insect Virome: Opportunities and Challenges

Introduction

The Drosophila virome

The mosquito virome

The honey bee virome

Implications of virus abundance in field populations

Viruses in insect cell lines

Future directions

2: The Widespread Occurrence and Potential Biological Roles of Endogenous Viral Elements in Insect Genomes

Introduction: definition of endogenous viral elements and the surprising discovery of those from non-retroviral RNA viruses

Integration of genomes from DNA viruses

Integration of non-retroviral RNA virus genomes: discovery and association with retroelements

Integration of non-retroviral RNA virus genomes: viral origin and number of NIRVS in insect genomes

Integration of RNA virus genomes: focus on Aedes spp.

Exogenous virus resistance mediated by endogenous viral elements

Endogenous viral elements and the piRNA pathway in mosquitoes

Concluding remarks and future directions

3: Sensing Viral Infections in Insects: A Dearth of Pathway Receptors

Antiviral immunity in insects

The siRNA pathway of RNA interference: mechanism and regulation

Control of viruses by the siRNA pathway

The piRNA pathway in antiviral immunity

Sensing viral RNA in insects

4: Micro-RNA Modulation of Insect Virus Replication

Introduction: insect-virus interaction and microRNAs

miRNA: genomics, biogenesis to regulation of target

RISC assembly and miRNA strand selection

miRNA roles in host-virus interaction

Host miRNA roles in insect-virus interactions

Virus miRNA roles in host-virus interaction

5: Dicistrovirus-Host Molecular Interactions

Viral entry, polyprotein processing and replication.

Unique translation mechanisms for dicistroviral protein synthesis

Host responses to viral infection

Pathogenesis

Biopesticides

Conclusions and future perspectives

6: Looking Through the Lens of 'Omics Technologies: Insights into the Transmission of Insect Vector-borne Plant Viruses

Advances in whole genome sequencing of insect vectors of plant viruses fuels discovery

Virus-vector protein interactions involved in non-circulative transmission

Virus-vector protein interactions involved in circulative, non-propagative transmission

Virus-vector interactions regulating circulative, propagative transmission

Host manipulation by plant viruses: an indirect strategy for promoting vector transmission

Vector manipulation by plant viruses: a direct strategy for promoting vector transmission

7: Advances in Tetravirus Research: New Insight into the Infectious Virus Life Cycle and an Expanding Host Range

Taxonomy

The tetravirus capsid

Genome organisation and regulation of viral gene expression

Elucidating the infectious virus life cycle

Redefining the host range of tetraviruses

Concluding remarks

8: Polydnaviruses: Evolution and Function

The early literature concluded that PDVs are beneficial symbionts of wasps

Key insights into PDV evolution and function required genomic data from wasps

BVs are endogenous virus elements (EVEs) that retain many ancestral functions

Other nudivirus-derived EVEs exist

9: Advances in the Molecular Biology of Baculoviruses

Baculovirus genome

PIF-mediated ODV entry into midgut cells (primary infection)

Envelope fusion protein (EFP)-mediated BV entry (systemic infection)

Essential functions of actin polymerization in baculovirus infection

Genome replication.

Viral gene transcription

Nucleocapsid assembly

BV budding and ODV morphogenesis

Regulation of host immunity, behaviour and physiology

Synthetic biology in baculoviruses

10: Recent Developments in the Use of Baculovirus Expression Vectors

Vector development

Incorporating RNAi technologies to improve protein production

Developments in cell lines to support recombinant protein production

Future developments

11: Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications

Surface-display platforms

Applications for baculoviral surface-display systems

Strategies for improvements

Summary and future outlook

Index.

Notes:

Description based on print version record.

ISBN:

1-912530-09-0

OCLC:

1103606020