Live cell assays : from research to health and regulatory applications / Christophe Furger.

Format:

Book

Author/Creator:

Furger, Christophe, author.

Series:

McGraw-Hill's biomedical engineering series.

Biomedical engineering series

Language:

English

Subjects (All):

Cells.

Biological assay.

Physical Description:

1 online resource (285 p.)

Edition:

1st ed.

Place of Publication:

Hoboken, New Jersey : ISTE Ltd/John Wiley and Sons Inc, 2016.

Summary:

Cell assays include all methods of measurements on living cells. Confined for a long time to research laboratories, these emerging methods have, in recent years, found industrial applications that are increasingly varied and, from now on, regulatory. Based on the recent explosion of knowledge in cell biology, the measurement of living cells represents a new class of industry-oriented research tests, the applications of which continue to multiply (pharmaceuticals, cosmetics, environment, etc.). Cellular tests are now being positioned as new tools at the interface between chemical methods, which are often obsolete and not very informative, and methods using animal models, which are expensive, do not fit with human data and are widely discussed from an ethical perspective. Finally, the development of cell assays is currently being strengthened by their being put into regulatory application, particularly in Europe through the REACH (Registration, Evaluation, Authorisation and Restriction of Chemicals) and cosmetic directives.

Contents:

Cover; Title Page; Copyright; Contents; Foreword; A researcher's view; A consultant's view; The author; Acknowledgments; Abbreviations; List of Cell Assays; Introduction; 1: Principles and Position; 1.1. Live cell assay principles; 1.2. Application areas; 1.3. Positioning; 1.3.1. Definition and typology of cell tests; 1.3.2. The regulatory and industrial dimension; 1.4. Market; 1.5. Competitive advantages; 1.5.1. Cells are live information models; 1.5.2. Development: high throughput; 1.5.3. Development: multiplex analysis; 1.5.4. Development: miniaturization

1.5.5. Development: molecular engineering1.5.6. Development: standardization; 1.6. Can measurements of cells in culture be extrapolated to effects in the organism?; 1.6.1. Toxicokinetics; 1.6.2. Components of the immune system; 1.6.3. Biotransformation; 1.6.4. The macrocellular environment; 1.7. Limits; 1.7.1. Importance of cellular microenvironment; 1.7.2. Other limits; 2: History and State of the Art; 2.1. Origins of cell culture; 2.1.1. Pioneering studies; 2.1.2. Alexis Carrel; 2.1.3. Were Dr Carrel's cells immortal?; 2.2. The HeLa line and the first applications of cell culture

2.2.1. A vaccine against poliomyelitis2.2.2. Cells in space; 2.2.3. Cell cloning; 2.3. New cell lines; 2.3.1. The CHO line; 2.3.2. An increasing number of cell lines; 2.4. Cross-contamination; 2.5. Cell lines, an ethical issue; 2.6. The first generation of cell assays (1969-1983); 2.6.1. The karyotype test; 2.6.2. The MTT assay; 2.6.3. The NRU test; 2.7. The first target of regulatory assays: genotoxicity (1983-1986); 2.7.1. Ames test (OECD guideline 471); 2.7.2. In vitro mammalian chromosome aberration test (OECD guideline 473)

2.7.3. In vitro mammalian cell gene mutation test (OECD guideline 476)2.7.4. In vitro sister chromatid exchange assay in mammalian cells (OECD guideline no. 479); 2.7.5. DNA damage and repair, unscheduled DNA synthesis in mammalian cells (OECD guideline 482); 3: Cell Models and Technologies; 3.1. Fluorescence and bioluminescence; 3.1.1. Green fluorescent protein; 3.1.2. BRET; 3.1.3. FRET; 3.1.4. Other applications of GFP; 3.1.5. The reporter gene approach; 3.2. Impedance variation in cell population; 3.3. Optical signals modified by state of cells; 3.4. Cellular autofluorescence

3.4.1. The case of chlorophyll3.5. The different cell models and culture modes available; 3.5.1. Immortalized lines; 3.5.2. Primary cells; 3.5.3. Three-dimensional cell culture; 4: Loss of Cell Homeostasis: Applications in Toxicity Measurement; 4.1. What relevant information to use in the living cell?; 4.2. Lysosomal activity; 4.3. Redox balance and oxidative stress; 4.4. Integrity of the plasma membrane; 4.5. Cellular efflux; 4.6. Homeostasis of ion exchanges; 4.6.1. The calcium ion; 4.6.2. Maintenance of membrane potential; 4.7. Metabolism and cell respiratory activity; 4.8. Genotoxicity

4.9. Apoptosis

Notes:

Description based upon print version of record.

Description based on print version record.

ISBN:

9781119330165

1119330165

9781119330172

1119330173

9781119330141

1119330149

OCLC:

956672082