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Function and regulation of the PDK1 kinase / Dario Alessi.

Henry Stewart Biomedical & Life Sciences Collection Available online

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Format:
Video
Author/Creator:
Alessi, Dario, speaker, author.
Series:
Henry Stewart talks. Biomedical & life sciences collection. Protein phosphorylation.
Protein phosphorylation, 2056-452X
Language:
English
Subjects (All):
Phosphorylation.
Protein Serine-Threonine Kinases.
Medical Subjects:
Phosphorylation.
Protein Serine-Threonine Kinases.
Genre:
Video recordings.
Physical Description:
1 online resource (1 streaming video file (58 min.)) : color, sound.
polychrome
Place of Publication:
London : Henry Stewart Talks, 2010.
System Details:
Mode of access: World Wide Web.
video file
Contents:
Contents: The insulin cascade
Approaches to study the insulin signalling pathway
GSK3 regulation of glycogen synthase by insulin
Inactivation of GSK3 by PI3K
GSK3 inactivation by other kinases
Protein Kinase B (Akt)
PKB signal transduction
Substrate specificity of PKB
Activation of PKB by insulin
Phosphorylation of PKB on Thr308 and Ser473
PKB phosphorylation on the catalytic domain
How insulin locates PKB to the plasma membrane
PKB translocation to the cell membrane
Molecular mechanism of PKB activation
Phosphorylation of PKB
Discovery of PDK1
Primary structure of human PDK1
PIP3 requirement for PDK1 activation of PKB
PDK1/2 phosphorylation of PKB
The inability of PIP3 to activate PDK1 in vitro
PKB in muscle-specific PDK1 knockout mice
Glycogen synthase inactivation by GSK3
GSK3 and glycogen synthase in muscle-specific PDK1 knockout mice
GSK3 knock-in mice
Analysis of GSK3 isoforms in knock-in mice
Glycogen synthase activity in knock-in mice
Insulin effects mediated by protein kinases
S6K, SGK and PKC activation
PDK1 as a "master upstream kinase"
S6K in muscle-specific PDK1 knockout mice
PDK1 activation of 24 AGC kinase members
Regulation of the PDK1 pathway
The PDK1 kinase domain
Defining the PIF-pocket by knock-in mutation
S6K in PDK1 PIF-pocket knock-in mutation
The role of PIP3 binding to the PH domain
Inhibition of PKB in PDK1 PH domain knock-in cells
The role of the PDK1 in cancer
Evaluation of PDK1 as a drug target
Generation of PDK1 hypomorphic mice
PTEN and its effect in cancer
PDK1 and PTEN in tumours.
Notes:
Animated audio-visual presentation with synchronized narration.
Title from title frames.
Publisher Number:
2827 Henry Stewart Talks
Access Restriction:
Restricted for use by site license.

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