Advances in Single Molecule, Real-Time (SMRT) Sequencing

Format:

Book

Author/Creator:

Ameur, Adam

Contributor:

Hestand, Matthew S.

Language:

English

Physical Description:

1 online resource (128 p.)

Other Title:

Advances in Single Molecule, Real-Time

Place of Publication:

MDPI - Multidisciplinary Digital Publishing Institute 2019

Language Note:

English

Summary:

PacBio's single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.

ISBN:

3-03921-701-1

Access Restriction:

Open Access Unrestricted online access