Mass Spectrometry: Developmental Approaches to Answer Biological Questions / by Gwenael Pottiez.

Format:

Book

Author/Creator:

Pottiez, Gwenael, 1982- author.

Contributor:

SpringerLink (Online service)

Series:

Biomedical and Life Sciences (Springer-11642)

SpringerBriefs in bioengineering 2193-097X

SpringerBriefs in Bioengineering, 2193-097X

Language:

English

Subjects (All):

Spectrum analysis.

Biochemical engineering.

Medicine.

Spectroscopy/Spectrometry.

Biochemical Engineering.

Biomedicine, general.

Local Subjects:

Spectroscopy/Spectrometry.

Biochemical Engineering.

Biomedicine, general.

Physical Description:

1 online resource (VIII, 71 pages) : 12 illustrations.

Edition:

First edition 2015.

Contained In:

Springer eBooks

Place of Publication:

Cham : Springer International Publishing : Imprint: Springer, 2015.

System Details:

text file PDF

Summary:

The understanding of the events taking place in a cell, a biological fluid or in any biological system is the main goal of biology research. Many fields of research use different technology to assess those events. Mass spectrometry is one of those techniques and this undergoes constant evolution and adaptation to always enhance the accuracy of the information provided. Proteomics provides a large panel of data on protein identity and protein expression that were made possible by mass spectrometry. For several years now mass spectrometry has become central to performing proteomic research, however this powerful tool is under constant evolution to be more sensitive and more resolute. More importantly mass spectrometry became a field of research focusing on new applications. Indeed, the complexity in biological systems relies on the changes of expression of transcription of proteins but also on the post-translational modification of proteins, the structure of proteins and the interaction between proteins, amongst others. As of now, several investigations tried to improve the quantification of proteins by mass spectrometry, the determination of post-translational modifications, the protein-protein and protein-nucleic acids interaction or the proteins structures. This book is structured as follows: after a brief introduction of the usual and most popular applications for mass spectrometry in proteomics, the most recent research and developments in mass spectrometry-based methodologies will be explored.

Contents:

General and most usual proteomics methods based on mass spectrometry analysis: Protein identification and protein quantification

Direct analysis of tissues and body fluids

Mass spectrometry in archaeology

Determination and quantification of post-translational modifications

Protein-Protein interaction determined by mass spectrometry

Protein structure analysis

Determination of protein function by mass spectrometry

Computer-assisted data analysis and data mining for new applications.

Other Format:

Printed edition:

ISBN:

978-3-319-13087-3

9783319130873

Access Restriction:

Restricted for use by site license.