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Immunohistochemistry : Essential Elements and Beyond / by Alexander E. Kalyuzhny.
- Format:
- Book
- Author/Creator:
- Kalyuzhny, Alexander E., author.
- Series:
- Biomedical and Life Sciences (Springer-11642)
- Techniques in life science and biomedicine for the non-expert 2367-1114
- Techniques in Life Science and Biomedicine for the Non-Expert, 2367-1114
- Language:
- English
- Subjects (All):
- Molecular biology.
- Immunoglobulins.
- Neurochemistry.
- Molecular Medicine.
- Antibodies.
- Local Subjects:
- Molecular Medicine.
- Antibodies.
- Neurochemistry.
- Physical Description:
- 1 online resource (XIV, 85 pages) : 36 illustrations, 2 illustrations in color.
- Edition:
- First edition 2016.
- Contained In:
- Springer eBooks
- Place of Publication:
- Cham : Springer International Publishing : Imprint: Springer, 2016.
- System Details:
- text file PDF
- Summary:
- Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: · producing transgenic animals · studying the effects of drug treatments · electrophysiological studies · animal surgery · isolating and differentiating stem cells · super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.
- Contents:
- Short Historic Overview
- Primary Antibodies
- Choosing Commercial Suppliers of Primary Antibodies
- Detection and Visualization of Antigens
- Ancillary Reagents for IHC
- Preparing Tissues for IHC Staining
- Staining Protocols
- Microscopy Examination and Collection of Digital Images and Their Presentation
- Concluding Remarks.
- Other Format:
- Printed edition:
- ISBN:
- 978-3-319-30893-7
- 9783319308937
- Access Restriction:
- Restricted for use by site license.
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