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Radiation Cytogenetics : Methods and Protocols / edited by Takamitsu A. Kato, Paul F. Wilson.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1064-3745 ; 1984.
- Methods in Molecular Biology, 1064-3745 ; 1984
- Language:
- English
- Subjects (All):
- Human genetics.
- Cell culture.
- Human Genetics.
- Cell Culture.
- Local Subjects:
- Human Genetics.
- Cell Culture.
- Physical Description:
- 1 online resource (X, 138 pages) : 18 illustrations, 11 illustrations in color.
- Edition:
- First edition 2019.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana, 2019.
- System Details:
- text file PDF
- Summary:
- This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.
- Contents:
- Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis
- Animal Lymphocyte Metaphase Chromosome Preparation
- Micronuclei Formation Analysis after Ionizing Radiation
- G1 Premature Chromosome Condensation (PCC) Assay
- G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity
- G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology
- Sister Chromatid Exchange as a Genotoxic Stress Marker
- DNA Damage Focus Formation Assay
- Nuclear Foci Assays in Live Cells
- In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes
- PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations
- Modified PNA Telomere and Centromere FISH Protocols
- Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements
- Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH
- Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-4939-9432-8
- 9781493994328
- Access Restriction:
- Restricted for use by site license.
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