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Immunological Tolerance : Methods and Protocols / edited by Ashleigh S. Boyd.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Boyd, Ashleigh S., editor.
SpringerLink (Online service)
Series:
Springer Protocols (Springer-12345)
Methods in molecular biology 1064-3745 ; 1899.
Methods in Molecular Biology, 1064-3745 ; 1899
Language:
English
Subjects (All):
Immunology.
Local Subjects:
Immunology.
Physical Description:
1 online resource (XI, 240 pages) : 43 illustrations, 36 illustrations in color.
Edition:
First edition 2019.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer New York : Imprint: Humana, 2019.
System Details:
text file PDF
Summary:
This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents:
Isolation and Characterisation of Human Adipose-derived Stem Cells
Isolation of Murine Hematopoietic Stem Cells
Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells
Isolation of Human Regulatory T Lymphocytes by Fluorescence-activated Cell Sorting
Identification and isolation of Regulatory B cells in Mouse and Human
An innovative Standard Operation Procedure for Isolating GMP-grade CD4+CD25+ T Cells from non-mobilized leukapheresis
Measuring CTLA-4-dependent Suppressive Function in Regulatory T Cells
Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation in vitro in the Context of Costimulatory Molecule Blockade
In Vitro Suppression of CD4+ T Cell Responses by Murine and Human Myeloid-derived Suppressor Cells
Disassembling and Re-aggregating the Thymus: The Plus and Minus of Current Assays
Isolation of Highly Viable Thymic Epithelial Cells for use in In Vitro and In Vivo Experiments
Immune tolerance and rejection in organ transplantation
Method of Generating Tolerogenic Maturation-resistant Dendritic Cells and Testing for their Immune-regulatory Functions In Vivo in the Context of Transplantation
Luminex Coupled-EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Autoreactive T Cells in Human Peripheral Blood
Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental
Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle. .
Other Format:
Printed edition:
ISBN:
978-1-4939-8938-6
9781493989386
Access Restriction:
Restricted for use by site license.

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