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Cryptosporidium : Methods and Protocols / edited by Jan R. Mead, Michael J. Arrowood.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1064-3745 ; 2052.
- Methods in Molecular Biology, 1064-3745 ; 2052
- Language:
- English
- Subjects (All):
- Parasitology.
- Microbiology.
- Infectious diseases.
- Infectious Diseases.
- Local Subjects:
- Parasitology.
- Microbiology.
- Infectious Diseases.
- Physical Description:
- 1 online resource (XI, 407 pages) : 93 illustrations, 71 illustrations in color.
- Edition:
- First edition 2020.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana, 2020.
- System Details:
- text file PDF
- Summary:
- This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon. The chapter "Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
- Contents:
- Cryptosporidium Diagnostic Assays: Microscopy
- Cryptosporidium Diagnostic Assays: Molecular Detection
- Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration
- Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation
- Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay
- Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii
- Cryptosporidium Genotyping for Epidemiology Tracking
- Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection
- Preparation of Cryptosporidium DNA for Whole Genome Sequencing
- Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org
- A Novel Method to Silence Genes in Cryptosporidium
- Use of miRNAs to Study Host Cell-Parasite Interactions
- Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9
- Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology
- Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics
- Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water
- High-Content Screening for Cryptosporidium Drug Discovery
- High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR
- In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds
- In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers
- Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-4939-9748-0
- 9781493997480
- Access Restriction:
- Restricted for use by site license.
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