Ion Mobility-Mass Spectrometry : Methods and Protocols / edited by Giuseppe Paglia, Giuseppe Astarita.

Format:

Book

Contributor:

Paglia, G. (Giuseppe), editor.

Astarita, Giuseppe, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 2084.

Methods in Molecular Biology, 1064-3745 ; 2084

Language:

English

Subjects (All):

Biochemistry.

Mass spectrometry.

Biochemistry, general.

Mass Spectrometry.

Local Subjects:

Biochemistry, general.

Mass Spectrometry.

Physical Description:

1 online resource (XI, 312 pages) : 102 illustrations, 83 illustrations in color.

Edition:

First edition 2020.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2020.

System Details:

text file PDF

Summary:

This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as an ideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.

Contents:

Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules

Utilizing Drift Tube Ion Mobility Spectrometry for the Evaluation of Metabolites and Xenobiotics

Untargeted Differential Metabolomics Analysis Using Drift Tube Ion Mobility-Mass Spectrometry

Drift Tube Ion Mobility-Mass Spectrometry for Non-Targeted 'Omics

The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines

Traveling Wave Ion Mobility-Mass Spectrometry to Enhance the Detection of Low Abundance Features in Untargeted Lipidomics

Lipidomics by HILIC-Ion Mobility-Mass Spectrometry

A Workflow for the Identification of Mycotoxin Metabolites Using Liquid Chromatography-Ion Mobility-Mass Spectrometry

Quantitation of Cyclosporin A in Cell Culture Media by Differential Mobility Mass Spectrometry (DMS-MS/MS)

An Analytical Perspective on Protein Analysis and Discovery Proteomics by Ion Mobility-Mass Spectrometry

Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics

Liquid Extraction Surface Analysis (LESA) High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Mass Spectrometry for In Situ Analysis of Intact Proteins

Ion Mobility-Mass Spectrometry of Glycoconjugates

Desorption Atmospheric Pressure Photoionization Coupled with Ion Mobility-Mass Spectrometry

Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation

Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility

The Use of LipidIMMS Analyzer for Lipid Identification in Ion Mobility-Mass Spectrometry-Based Untargeted Lipidomics

MolFind2: A Protocol for Acquiring and Integrating MS3 Data to Improve In-Silico Chemical Structure Elucidation for Metabolomics

Collision Cross Section Calculations Using HPCCS.

Other Format:

Printed edition:

ISBN:

978-1-0716-0030-6

9781071600306

Access Restriction:

Restricted for use by site license.