Viral Vectors for Gene Therapy : Methods and Protocols / edited by Fredric P. Manfredsson, Matthew J. Benskey.

Format:

Book

Contributor:

Manfredsson, Fredric P., editor.

Benskey, Matthew J., editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1937.

Methods in Molecular Biology, 1064-3745 ; 1937

Language:

English

Subjects (All):

Gene therapy.

Virology.

Gene Therapy.

Local Subjects:

Gene Therapy.

Virology.

Physical Description:

1 online resource (XIV, 328 pages) : 46 illustrations, 33 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Viral Vectors for Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments.

Contents:

Basic Concepts in Viral Vector-Mediated Gene Therapy

Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing

Design, Construction, and Application of Transcription Activation-Like Effectors

Practical Considerations for the use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain

AAV Production using Baculovirus Expression Vector System

Multimodal Production of Adeno-Associated Virus

Generation of High Titer Pseudotyped Lentiviral Particles

A Scalable Lentiviral Vector Production and Purification Method using Mustang Q Chromatography and Tangential Flow Filtration

Current Use of Adenovirus Vectors and their Purification Methods

Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest

Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes

AAV Mediated Gene Delivery to the Mouse Liver

Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse

Gene Transfer to Mouse Kidney In Vivo

Co-Delivery of a Short-Hairpin RNA and a shRNA-Resistant Replacement Gene with Adeno-Associated Virus: An Allele-Independent Strategy for Autosomal Dominant Retinal Disorders

Localized Intra-Arterial Gene Delivery using AAV

Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors

Systemic Delivery of Adeno-Associated Viral Vectors in Mice and Dogs

Intrathecal Delivery of AAV Vectors in Cynomolgus Macaques for CNS Gene Therapy and Gene Expression Analysis in Microdissected Motor Neurons

Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice

Cerebromedullary Cistern Injection of Viral Vectors in Non-Human Primates.

Other Format:

Printed edition:

ISBN:

978-1-4939-9065-8

9781493990658

Access Restriction:

Restricted for use by site license.