Immune Checkpoint Blockade : Methods and Protocols / edited by Yago Pico de Coaña.

Format:

Book

Contributor:

Pico de Coaña, Yago, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1913.

Methods in Molecular Biology, 1064-3745 ; 1913

Language:

English

Subjects (All):

Cancer--Research.

Cancer.

Immunology.

Cancer Research.

Local Subjects:

Cancer Research.

Immunology.

Physical Description:

1 online resource (XI, 225 pages) : 50 illustrations, 35 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies.

Contents:

Double Immunohistochemistry and Digital Image Analysis

Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells

Systems Level Immune Monitoring by Mass Cytometry

Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry

High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry

Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification

Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors

Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies

Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes

Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry

Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro

Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay

Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry

Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models

Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice

Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry.

Other Format:

Printed edition:

ISBN:

978-1-4939-8979-9

9781493989799

Access Restriction:

Restricted for use by site license.