Calcium-Binding Proteins of the EF-Hand Superfamily : From Basics to Medical Applications / edited by Claus W. Heizmann.

Format:

Book

Contributor:

Heizmann, Claus W., editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1929.

Methods in Molecular Biology, 1064-3745 ; 1929

Language:

English

Subjects (All):

Proteins.

Diagnosis, Laboratory.

Protein Science.

Laboratory Medicine.

Local Subjects:

Protein Science.

Laboratory Medicine.

Physical Description:

1 online resource (XIX, 779 pages) : 175 illustrations, 117 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.

Contents:

Using Data Mining to Explore Calmodulin Bibliography

Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator

High Resolution Calcium Imaging Method for Local Calcium Signaling

Measurement of Contractility and Calcium Release in Cardiac Spheroids

Simultaneous Recording of Subcellular Ca2+ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis

The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca2+ Signalling Events during the Early Development of Zebrafish (Danio rerio)

Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements

Designing Calcium Binding Proteins for Molecular MR Imaging

Coordination to Divalent Cations by Calcium Binding Proteins

Chaperoning against Amyloid Aggregation: Monitoring In Vitro and In Vivo

Ca2+-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets

Gene Transfer of Calcium Binding Proteins into Adult Cardiac Myocytes

Expression and Purification of Calmodulin for NMR and Other Biophysical Applications

The Use of Cre-loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signalling in Myeloid Cells

Calpain Purification through Calpastatin and Calcium: Strategy and Procedures

Characterization of the EF-Hand Calcium-Binding Domains of Human Plastins

Expression and Characterization of MICU2, a Ca2+ Sensor Protein

S100 Proteins in the Innate Immune Response to Pathogens

Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors

Monitoring Interactions between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy

Isolation and Characterization of S100 Protein-Protein Complexes

The Multifaceted S100A4 Protein in Cancer and Inflammation

Interaction of S100A6 with Target Proteins In Vitro and in Living Cells

Preparation of the Oxidized and Reduced Forms of Psoriasin (S100A7)

Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin

Structural Analysis of S100A8 Complex with Zinc and Calcium: A General Protocol for the Study of S100 Proteins in the Presence of Divalent Cations by X-Ray Crystallography

Analysis of Ca2+-Dependent Weibel-Palade Body Tethering by Live Cell TIRF Microscopy: Involvement of a Munc13-4/S100A10/Annexin A2 Complex

Analysis of S100A11 in DNA Damage Repair

Fluorine-18 Labeling of S100 Proteins for Small Animal Positron Emission Tomography

Reviewing the Crystal Structure of S100Z and Other Members of the S100 Family: Implications in Calcium-Regulated Quaternary Structure

High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins

Calcium-Induced Protein Folding in Calumenin and Calmodulin

Measuring Calumenin Impact on ER-Calcium Depletion Using Transient Calumenin Overexpression and Silencing

Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies

Tryptophan Scanning Mutagenesis of EF-Hand Motifs

Mapping Calcium-Sensitive Regions in GCAPs by Site-Specific Fluorescence Labeling

Quantification of Human Swiprosin-1/EFhd2 Expression on Protein and RNA Level

Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation

Characterization of Calcium-Binding Proteins from Parasitic Worms

High-Sensitivity Troponin Assays in Clinical Diagnostics of Acute Coronary Syndrome

Targeted Mass Spectrometry of S100 Proteins

Clinical Use of the Calcium-Binding S100B Protein, a Biomarker for Head Injury

Serum S100B Levels in Melanoma

The Ca2+-Binding S100B Protein: An Important Diagnostic and Prognostic Neurobiomarker in Pediatric Laboratory Medicine

S100A7 in Psoriasis: Immunodetection and Activation by CRISPR

S100A8/A9 in Myocardial Infarction

Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults

S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions.

Other Format:

Printed edition:

ISBN:

978-1-4939-9030-6

9781493990306

Access Restriction:

Restricted for use by site license.