Glutamate Receptors : Methods and Protocols / edited by Corinna Burger, Margaret Jo Velardo.

Format:

Book

Contributor:

Burger, Corinna, editor.

Velardo, Margaret Jo, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1941.

Methods in Molecular Biology, 1064-3745 ; 1941

Language:

English

Subjects (All):

Neurosciences.

Local Subjects:

Neurosciences.

Physical Description:

1 online resource (X, 260 pages) : 67 illustrations, 48 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications.

Contents:

Single Molecule FRET Methods to Study Glutamate Receptors

Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM)

Considerations for Imaging and Analyzing Neural Structures by STED Microscopy

Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors

Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function

Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE)

Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus

Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats

Whole-Cell Patch Clamp Electrophysiology to Study Inotropic Glutamatergic Receptors and Their Roles in Addiction

Gene Expression Analysis by Multiplex Single Cell RT-PCR

Metabolomics Analysis of Glutamate Receptor Function

Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation

Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function

Fractionation of Subcellular Compartments from Human Brain Tissue

Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection.

Other Format:

Printed edition:

ISBN:

978-1-4939-9077-1

9781493990771

Access Restriction:

Restricted for use by site license.