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Alpha-Synuclein : Methods and Protocols / edited by Tim Bartels.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1064-3745 ; 1948.
- Methods in Molecular Biology, 1064-3745 ; 1948
- Language:
- English
- Subjects (All):
- Proteins.
- Neurosciences.
- Protein Science.
- Local Subjects:
- Protein Science.
- Neurosciences.
- Physical Description:
- 1 online resource (XII, 289 pages) : 66 illustrations, 55 illustrations in color.
- Edition:
- First edition 2019.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana, 2019.
- System Details:
- text file PDF
- Summary:
- This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects.
- Contents:
- Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons
- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue
- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons
- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform
- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils
- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection
- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers
- Studying α-Synuclein Conformation by Intact-Cell Crosslinking
- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques
- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein
- Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes
- Yeast-Based Screens to Target Alpha-Synuclein Toxicity
- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy
- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers
- Monitoring α-Synuclein Proteotoxicity in Drosophila Models
- Detection of α-Synuclein in Biological Samples using Mass Spectrometry
- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation
- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons
- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy
- Expression and Purification of Untagged α-Synuclein
- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson's Disease.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-4939-9124-2
- 9781493991242
- Access Restriction:
- Restricted for use by site license.
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