Experimental Cholestasis Research / edited by Mathieu Vinken.

Format:

Book

Contributor:

Vinken, Mathieu, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1981.

Methods in Molecular Biology, 1064-3745 ; 1981

Language:

English

Subjects (All):

Pharmacology.

Hepatology.

Pharmacology/Toxicology.

Local Subjects:

Pharmacology/Toxicology.

Hepatology.

Physical Description:

1 online resource (XIII, 399 pages) : 74 illustrations, 60 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology.

Contents:

Mechanisms of Drug-Induced Cholestasis

LC-MS/MS Analysis of Bile Acids in In Vitro Samples

Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis

Vesicle-Based and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function

The C-DILI™ Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity

In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids

Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis

Measuring Apoptosis and Necrosis in Cholestatic Liver Injury

Necrosome Formation and Necroptosis in Experimental Cholestasis

Proteomics in Primary Biliary Cholangitis

Transcriptomics Analysis of Cholestatic Compounds In Vitro

Systems Microscopy of Stress Response Pathways in Cholestasis Research

Mouse Models for Diseases in the Cholangiocyte Lineage

Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis

3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research

A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis

Zebrafish as a Model to Study Cholestatic Liver Diseases

Set-Up and Use of HepaRG Cells in Cholestasis Research

Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced Cholestasis

Establishment of Sandwich Cultures of Primary Human Hepatocytes

Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes

Ex Vivo Model in Cholestasis Research

Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies

Use of Biliary Organoids in Cholestasis Research

In Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data Mining, Model Generation, and Link to Cholestasis.

Other Format:

Printed edition:

ISBN:

978-1-4939-9420-5

9781493994205

Access Restriction:

Restricted for use by site license.