Collagen : Methods and Protocols / edited by Irit Sagi, Nikolaos A. Afratis.

Format:

Book

Contributor:

Sagi, Irit, editor.

Afratis, Nikolaos A., editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1944.

Methods in Molecular Biology, 1064-3745 ; 1944

Language:

English

Subjects (All):

Proteins.

Cytology.

Protein Science.

Cell Biology.

Local Subjects:

Protein Science.

Cell Biology.

Physical Description:

1 online resource (XI, 254 pages) : 60 illustrations, 44 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.

Contents:

Cloning and Mutagenesis Strategies for Large Collagens

Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen

Production and Characterization of Chemically Cross-Linked Collagen Scaffolds

A Biomimetic Model for Mineralization of Type-I Collagen Fibrils

Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy

Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry

Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes

Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics

Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy

In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology

Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics

Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix

Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans

Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis

Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment

FACS Analysis of Col1α Protein Levels in Primary Fibroblasts

Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors.

Other Format:

Printed edition:

ISBN:

978-1-4939-9095-5

9781493990955

Access Restriction:

Restricted for use by site license.