Amino Acid Analysis : Methods and Protocols / edited by Michail A. Alterman.

Format:

Book

Contributor:

Alterman, Michail A., editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 2030.

Methods in Molecular Biology, 1064-3745 ; 2030

Language:

English

Subjects (All):

Biochemistry.

Mass spectrometry.

Biochemistry, general.

Mass Spectrometry.

Local Subjects:

Biochemistry, general.

Mass Spectrometry.

Physical Description:

1 online resource (XVII, 455 pages) : 104 illustrations, 45 illustrations in color.

Edition:

Second edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs. In this volume, readers can find chapters describing general, as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry, as well as in food analysis, microbiology, marine biology, drug metabolism, even archaeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols, Second Edition provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Contents:

Ultra-Sensitive Quantification of Recombinant Proteins using AAA-MS

Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry

Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass Spectrometry

Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content

Analysis of Hydroxyproline in Collagen Hydrolysates

Quantification of Isotopologues of Amino Acids by Multiplexed Stable Isotope Resolved Metabolomics using Ultrahigh Resolution Mass Spectrometry Coupled with Direct Infusion

Preparative HPLC Separation of Underivatized Amino Acids for Isotopic Analysis

Quantitative Analysis of Underivatized Amino Acids by Liquid Chromatography-Tandem Mass Spectrometry

Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass Spectrometry

The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determination of Plasma Tryptophan and other Amino Acids

Analysis of Tryptophan and Its Metabolites by High-Performance Liquid Chromatography

Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis

Quantification of Underivatized Amino Acids on Dry Blood Spot, Plasma, and Urine by HPLC-ESI-MS/MS

Amino Acid Analysis in Physiological Samples by GC-MS with Propylchloroformate Derivatization and iTRAQ-LC-MS/MS

Combination of an AccQ·Tag-Ultra Performance Liquid Chromatographic Method with Tandem Mass Spectrometry for the Analysis of Amino Acids

Methods for Absolute Quantification of Human Plasma Free Amino Acids by High-Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry using Precolumn Derivatization

Analysis and Enantioseparation of Amino Acids by Liquid Chromatography

Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Chiral and Nonchiral Amino Acid Analysis by Gas Chromatography

High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis without Derivatization

A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids

Chiral Analysis of Non-Protein Amino Acids by Capillary Electrophoresis

Amino Acid Analysis via LC-MS Method after Derivatization with Quaternary Phosphonium

Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry

Capillary Electrophoresis of Free Amino Acids in Physiological Fluids without Derivatization Employing Direct or Indirect Absorbance Detection

New Advances in Amino Acid Profiling in Biological Samples by Capillary Electrophoresis-Mass Spectrometry

Enhanced Separation Capability of Sequential Injection Chromatography for Fluorimetric Determination of Intracellular Dissolved Free Amino Acids in Marine Microalgae

Sensitive and Selective Amino Acid Profiling of Minute Tissue Amounts by HPLC/Electrospray Negative Tandem Mass Spectrometry using 9-Fluorenylmethoxycarbonly (Fmoc-Cl) Derivatization

Wheat Gluten Amino Acid Analysis by High-Performance Anion-Exchange Chromatography with Integrated Pulsed Amperometric Detection

Analysis of Underivatized Amino Acids: Zwitterionic Hydrophilic Interaction Chromatography Combined with Triple Quadrupole Tandem Mass Spectrometry

Direct Analysis of Underivatized Amino Acids in Plant Extracts by LC-MS-MS (Improved Method)

Optimal Conditions for the Direct RP-HPLC Determination of Underivatized Amino Acids with On-Line Multiple Detection

Non-Marking Collection of Amino Acids from Fingerprints using Hydrogels

Amino Acid Profiling from Fingerprints using Amide Stationary Phase UPLC-MS.

Other Format:

Printed edition:

ISBN:

978-1-4939-9639-1

9781493996391

Access Restriction:

Restricted for use by site license.