Genotype Phenotype Coupling : Methods and Protocols / edited by Stefan Zielonka, Simon Krah.

Format:

Book

Contributor:

Zielonka, Stefan, editor.

Krah, Simon, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 2070.

Methods in Molecular Biology, 1064-3745 ; 2070

Language:

English

Subjects (All):

Human genetics.

Proteins.

Human Genetics.

Protein Science.

Local Subjects:

Human Genetics.

Protein Science.

Physical Description:

1 online resource (XII, 445 pages) : 86 illustrations, 61 illustrations in color.

Edition:

First edition 2020.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2020.

System Details:

text file PDF

Summary:

This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field. .

Contents:

Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin

Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins

cDNA Display: A Stable and Simple Genotype-phenotype Coupling Using a Cell-free Translation System

cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution

Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display

Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library

In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties

Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens

Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning

Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display

Selection and Characterization of Anti-idiotypic Shark Antibody Domains

Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression

Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors

Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting

Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires

Rapid Generation of Chicken Immune Libraries for Yeast Surface Display

Ligand Engineering via Yeast Surface Display and Adherent Cell Panning

Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping

Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies

Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors

Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets

Engineering Antibodies on the Surface of CHO Cells

Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies.

Other Format:

Printed edition:

ISBN:

978-1-4939-9853-1

9781493998531

Access Restriction:

Restricted for use by site license.