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Antibody-Drug Conjugates : Methods and Protocols / edited by L. Nathan Tumey.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1064-3745 ; 2078.
- Methods in Molecular Biology, 1064-3745 ; 2078
- Language:
- English
- Subjects (All):
- Immunoglobulins.
- Pharmacology.
- Antibodies.
- Pharmacology/Toxicology.
- Local Subjects:
- Antibodies.
- Pharmacology/Toxicology.
- Physical Description:
- 1 online resource (XI, 373 pages) : 138 illustrations, 109 illustrations in color.
- Edition:
- First edition 2020.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2020.
- System Details:
- text file PDF
- Summary:
- This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the "activation barrier" when undertaking a new discipline, and provides a "toolbox" for the next generation of ADC scientists.
- Contents:
- An Overview of the Current ADC Discovery Landscape
- Pushing the Envelope: Advancement of ADCs Outside of Oncology
- Conjugations to Endogenous Cysteine Residues
- Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies
- Transglutaminase-Mediated Conjugations
- Click Chemistry Conjugations
- Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates
- Bridged Cysteine Conjugations
- Antibody Conjugations via Glycosyl Remodeling
- ADC Analysis by Hydrophobic Interaction Chromatography
- Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs
- Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction
- Analysis of ADCs by Native Mass Spectrometry
- High Resolution Characterization of ADCs by Orbitrap LCMS
- Conjugation Site Analysis by MS/MS Protein Sequencing
- Conjugation Site Analysis of Lysine-Conjugated ADCs
- Characterization of ADCs by Capillary Electrophoresis
- Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry
- Purification of ADCs by Hydrophobic Interaction Chromatography
- Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations
- Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions
- Biophysical Methods for Characterization of Antibody Drug Conjugates
- Determination of ADC Cytotoxicity in Immortalized Human Cell Lines
- LC/MS Methods for Studying Lysosomal ADC Catabolism
- Assessing ADC Plasma Stability by LC/MS Methods
- Determination of ADC Concentration by Ligand-Binding Assays.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-4939-9929-3
- 9781493999293
- Access Restriction:
- Restricted for use by site license.
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