Purinergic Signaling : Methods and Protocols / edited by Pablo Pelegrín.

Format:

Book

Contributor:

Pelegrín, Pablo, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 2041.

Methods in Molecular Biology, 1064-3745 ; 2041

Language:

English

Subjects (All):

Cytology.

Cell Biology.

Local Subjects:

Cell Biology.

Physical Description:

1 online resource (XII, 360 pages) : 82 illustrations, 49 illustrations in color.

Edition:

First edition 2020.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2020.

System Details:

text file PDF

Summary:

This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.

Contents:

Introduction to Purinergic Signaling

Knock-out and Knock-in Mouse Models to Study Purinergic Signaling

Agonists and Antagonists for Purinergic Receptors

Homology Modelling of P2X Receptors

Using RNA Interference for Purinoceptor Knock Down in vivo

Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos

Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues

Flow Cytometry of Membrane Purinoreceptors

Studying Purinoceptor Cell-surface Expression by Protein Biotinylation

Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking

Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer

Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy

Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor

Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse

Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging

Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels

ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader

P2X Electrophysiology and Surface Trafficking in Xenopus oocytes

Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells

Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages

Automated Planar Patch-clamp Recording of P2X Receptors

Controlling Engineered P2X Receptors with Light

Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment

Assays to Measure Purinoceptor Pore Dilation

Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization

Measuring Leukocyte Migration to Nucleotides

Assessment of Cell Adhesion after Purinoceptor Activation.

Other Format:

Printed edition:

ISBN:

978-1-4939-9717-6

9781493997176

Access Restriction:

Restricted for use by site license.