MAIT Cells : Methods and Protocols / edited by Helen Kaipe, Isabelle Magalhaes.

Format:

Book

Contributor:

Kaipe, Helen, editor.

Magalhaes, Isabelle, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 2098.

Methods in Molecular Biology, 1064-3745 ; 2098

Language:

English

Subjects (All):

Immunology.

Cytology.

Cell Biology.

Local Subjects:

Immunology.

Cell Biology.

Physical Description:

1 online resource (XII, 310 pages) : 54 illustrations, 41 illustrations in color.

Edition:

First edition 2020.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2020.

System Details:

text file PDF

Summary:

This volume focuses on various methods used by researchers to study mucosal-associated invariant T (MAIT) cells in the aspects described below. The chapters are organized into Four Parts: part One looks at the function and importance of MAIT cells in health and disease. It covers methods to isolate and characterize MAIT cells from human tissues including liver, colon tumors, placenta and decidua, and endometrium. Part Two discusses MR1 independent stimulation, and looks at the activation of MAIT cells by different stimulatory agents. Part Three describes various methods to develop tools to analyze, monitor, and generate MAIT cells in vitro, including the production of MR1-tetramers and how they can be loaded with bacterial antigens and used for the detection of MAIT cells. The last part covers the use of murine models to study MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, MAIT Cells: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field. Chapters 9 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

MAIT Cells in Health and Disease

Isolation and Characterization of MAIT Cells from Human Tissue Biopsies

Isolation and Characterization of MAIT Cells from Tumor Tissues

Isolation of Immune Cells from Placental Tissues and Phenotypic and Functional Analysis of MAIT Cells

Methods for High-Dimensional Flow Cytometry Analysis of Human MAIT Cells in Tissues and Peripheral Blood

In Situ Detection of MAIT Cells and MR1 Expressing Cells in Tissue Biopsies Utilizing Immunohistochemistry

Human MAIT Cell Activation In Vitro

Interactions between MAIT Cells and Dendritic Cells

Influenza A Virus-Infected Lung Epithelial Cell Co-Culture with Human Peripheral Blood Mononuclear Cells

Quantification of Human MAIT Cell-Mediated Cellular Cytotoxicity and Antimicrobial Activity

MAIT Cell Activation by Fungal Pathogens

A Flow Chamber Assay for Studying MAIT Cell Trafficking

Production of MR1 Tetramers Loaded with Microbial Ligands

Immune Monitoring of Human Mucosal Associated Invariant T Cells by Quantitative Proteomics

Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells

Reprogramming of MAIT Cells to Pluripotency and Redifferentiation

Study of MAIT Cell Activation in Viral Infections In Vivo

MAIT Cells in Type 1 Diabetes Mouse Models

Magnetic-Bead Based Enrichment of Murine MAIT Cells.

Other Format:

Printed edition:

ISBN:

978-1-0716-0207-2

9781071602072

Access Restriction:

Restricted for use by site license.