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High-Throughput Protein Production and Purification : Methods and Protocols / edited by Renaud Vincentelli.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1064-3745 ; 2025.
- Methods in Molecular Biology, 1064-3745 ; 2025
- Language:
- English
- Subjects (All):
- Proteins.
- Protein Science.
- Local Subjects:
- Protein Science.
- Physical Description:
- 1 online resource (XVI, 537 pages) : 145 illustrations, 106 illustrations in color.
- Edition:
- First edition 2019.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana, 2019.
- System Details:
- text file PDF
- Summary:
- This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.
- Contents:
- Part 1: General procedures for high-throughput protein production
- Overview of high-throughput cloning methods for the post-genomic era
- Overview of a high-throughput pipeline for streamlining the production of recombinant proteins
- Semi-automated small-scale purification method for high-throughput expression analysis of recombinant proteins
- High-Throughput Protein Production in Yeast
- A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells
- A High-throughput Automated Protein Folding System
- Part 2: High-throughput Protocols adapted to the production of specific protein families
- High-throughput production of oxidized animal toxins in Escherichia coli
- High-Throughput purification of protein kinases in Escherichia coli and insect cells
- Parallelized micro-scale expression of soluble scFv
- High throughput production of influenza virus-like particle (VLP) array by using VLP-factoryTM, a MultiBac baculoviral genome customized for enveloped VLP expression
- High-throughput Protein Production of membrane proteins in Saccharomyces cerevisiae
- High-throughput E. coli cell-free expression: From PCR product design to functional validation of GPCR
- High-Throughput Site-Directed Mutagenesis
- Part 3: High-throughput protein production combined with High-throughput micro-characterization for protein stability, solubility, quality and interactions
- Hot CoFi blot - A High-throughput colony-based screen for identifying more thermally stable protein variants
- High-throughput isolation of soluble protein domains using the bipartite split-GFP system
- High-throughput Analytical Light Scattering for Protein Quality Control and Characterization
- High-throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography
- A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production
- Adaption of the Leishmania cell-free expression system to high-throughput analysis of protein interactions
- High-throughput protein-protein interaction assays using tripartite split-GFP complementation
- High-throughput production of a new library of human single and tandem PDZ domains allows quantitative PDZ-peptide interaction screening through high throughput holdup assay
- High throughput protein analysis using Negative Stain Electron Microscopy and 2D classification
- High-throughput protein production combined with High throughput SELEX identifies an extensive atlas of Ciona robusta transcription factor DNA-binding specificities
- High-throughput Micro-characterization of RNA-protein interactions.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-4939-9624-7
- 9781493996247
- Access Restriction:
- Restricted for use by site license.
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