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Chlamydia trachomatis : Methods and Protocols / edited by Amanda Claire Brown.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1064-3745 ; 2042.
- Methods in Molecular Biology, 1064-3745 ; 2042
- Language:
- English
- Subjects (All):
- Medical microbiology.
- Infectious diseases.
- Pharmaceutical technology.
- Bacteriology.
- Medical Microbiology.
- Infectious Diseases.
- Pharmaceutical Sciences/Technology.
- Local Subjects:
- Medical Microbiology.
- Infectious Diseases.
- Pharmaceutical Sciences/Technology.
- Bacteriology.
- Physical Description:
- 1 online resource (XI, 289 pages) : 33 illustrations, 26 illustrations in color.
- Edition:
- First edition 2019.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana, 2019.
- System Details:
- text file PDF
- Summary:
- This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.
- Contents:
- Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA)
- Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue
- Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae
- Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay
- Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth
- Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples
- Multi Locus Sequence Typing (MLST) of Chlamydiales
- Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method
- Dual RNA-Seq of Chlamydia and Host Cells
- Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection
- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions
- Mutagenesis of Chlamydia trachomatis Using TargeTron
- Forward and Reverse Genetic Analysis of Chlamydia
- A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions
- Rodent Infections for Chlamydia spp.
- Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy
- Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane
- High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-4939-9694-0
- 9781493996940
- Access Restriction:
- Restricted for use by site license.
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