Neuroproteomics / edited by Ka Wan Li.

Format:

Book

Contributor:

Li, Ka Wan, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Neuromethods 0893-2336 ; 146.

Neuromethods, 0893-2336 ; 146

Language:

English

Subjects (All):

Neurosciences.

Proteomics.

Local Subjects:

Neurosciences.

Proteomics.

Physical Description:

1 online resource (XIII, 218 pages) : 55 illustrations, 37 illustrations in color.

Edition:

Second edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms.

Contents:

Neuroproteomics: The Methods

Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue

Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice

Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue

High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics

Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3)

Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip

Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes

Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry

TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons

Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures

Quantitative Analysis of Mass Spectrometry-Based Proteomics Data

2D-DIGE as a Tool in Neuroproteomics

Neuropeptidomics of the Mammalian Brain

MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides

A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder.

Other Format:

Printed edition:

ISBN:

978-1-4939-9662-9

9781493996629

Access Restriction:

Restricted for use by site license.