Oligodendrocytes : Methods and Protocols / edited by David A. Lyons, Linde Kegel.

Format:

Book

Contributor:

Lyons, David A., editor.

Kegel, Linde, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1936.

Methods in Molecular Biology, 1064-3745 ; 1936

Language:

English

Subjects (All):

Cytology.

Neurosciences.

Cell Biology.

Local Subjects:

Cell Biology.

Neurosciences.

Physical Description:

1 online resource (XII, 411 pages) : 65 illustrations, 55 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.

Contents:

Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System

Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing

Myelin: Methods for Purification and Proteome Analysis

In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells

Isolation and Culture of Oligodendrocytes

A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation

Oligodendrocyte-Neuron Myelinating Co-Culture

Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier

Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices

Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord

Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination

Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination

A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation

Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis

Conditional Mutagenesis in Oligodendrocyte Lineage Cells

Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage

The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination

Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease

A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes

Transmission Electron Microscopy of Oligodendrocytes and Myelin

Toxin-Based Models to Investigate Demyelination and Remyelination

Magnetic Resonance techniques for Imaging White Matter.

Other Format:

Printed edition:

ISBN:

978-1-4939-9072-6

9781493990726

Access Restriction:

Restricted for use by site license.