Neisseria meningitidis : Methods and Protocols / edited by Kate L. Seib, Ian R. Peak.

Format:

Book

Contributor:

Seib, Kate L., editor.

Peak, Ian R., editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1969.

Methods in Molecular Biology, 1064-3745 ; 1969

Language:

English

Subjects (All):

Bacteriology.

Vaccines.

Infectious diseases.

Vaccine.

Infectious Diseases.

Local Subjects:

Bacteriology.

Vaccine.

Infectious Diseases.

Physical Description:

1 online resource (XIII, 253 pages) : 58 illustrations, 42 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen.

Contents:

An Overview of Neisseria meningitides

Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR

Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis

Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics

Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis

Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis

Sample Preparation for Proteomic Analysis of Neisseria meningitides

Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance

The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.

In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells

Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease

Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays

Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines

Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine

Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides

Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis.

Other Format:

Printed edition:

ISBN:

978-1-4939-9202-7

9781493992027

Access Restriction:

Restricted for use by site license.