TRP Channels : Methods and Protocols / edited by Antonio Ferrer-Montiel, Tim Hucho.

Format:

Book

Contributor:

Ferrer-Montiel, Antonio, editor.

Hucho, Tim, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1987.

Methods in Molecular Biology, 1064-3745 ; 1987

Language:

English

Subjects (All):

Biochemistry.

Pharmaceutical technology.

Biochemistry, general.

Pharmaceutical Sciences/Technology.

Local Subjects:

Biochemistry, general.

Pharmaceutical Sciences/Technology.

Physical Description:

1 online resource (X, 238 pages) : 69 illustrations, 58 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab. Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels.

Contents:

Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels

Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6

Cryo-Electron Microscopy of TRP Channels

Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation

Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels

Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique

Fluorescence-Based Functional Assays for Ca2+ Permeable ThermoTRP Channels

High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons

Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels

TRP Channels Reconstitution in Lipid Bilayers

Methods for Investigating TRP Channel Gating

In Silico Approaches for TRP Channel Modulation

Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction

In Vivo Methods to Study ThermoTRP Channels in Rodents.

Other Format:

Printed edition:

ISBN:

978-1-4939-9446-5

9781493994465

Access Restriction:

Restricted for use by site license.