Cell Culture Techniques / edited by Michael Aschner, Lucio Costa.

Format:

Book

Contributor:

Aschner, Michael, 1955- editor.

Costa, Lúcio, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Neuromethods 0893-2336 ; 145.

Neuromethods, 0893-2336 ; 145

Language:

English

Subjects (All):

Neurosciences.

Cell culture.

Cell Culture.

Local Subjects:

Neurosciences.

Cell Culture.

Physical Description:

1 online resource (XIII, 302 pages) : 57 illustrations, 47 illustrations in color.

Edition:

Second edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.

Contents:

In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model

In Vitro Techniques for Assessing Neurotoxicity using Human iPSC-Derived Neuronal 1 Models

Oxidative Stress Signatures in Human Stem Cell Derived Neurons

Glial Reactivity in Response to Neurotoxins: Relevance and Methods

Neuron-Glia Interaction Studied with In Vitro Co-Culture

Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria

Epigenetic Changes in Cultured Neurons and Astrocytes

The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation

Zebrafish as a Tool to Assess Developmental Neurotoxicity

Rat Brain Slices: An Optimum Biological Preparation for acute Neurotoxicological Studies

Electrophysiological Neuromethodologies

A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies

Transporter Studies: Brain Punching Technique

MicroRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders

Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes.

Other Format:

Printed edition:

ISBN:

978-1-4939-9228-7

9781493992287

Access Restriction:

Restricted for use by site license.