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Snake and Spider Toxins : Methods and Protocols / edited by Avi Priel.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Priel, Avi, editor.
SpringerLink (Online service)
Series:
Springer Protocols (Springer-12345)
Methods in molecular biology 1064-3745 ; 2068.
Methods in Molecular Biology, 1064-3745 ; 2068
Language:
English
Subjects (All):
Pharmacology.
Pharmacology/Toxicology.
Local Subjects:
Pharmacology/Toxicology.
Physical Description:
1 online resource (X, 293 pages) : 46 illustrations, 32 illustrations in color.
Edition:
First edition 2020.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer US : Imprint: Humana, 2020.
System Details:
text file PDF
Summary:
This volume explores techniques and protocols, across various biological disciplines, used to study snake and spider peptide toxins. The chapters in this book are organized into four parts and cover topics such as the use of toxins in drug development; analysis of bioactivity of complex mixtures like venom; extraction of venom glands and the production of toxins; characterization of toxins from the RNA level to the peptide structure; and the determination of the toxin's biological function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Snake and Spider Toxins: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. .
Contents:
Snake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development
Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms
Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions ("Milking") and Venom Gland Extractions
Production and Purification of Recombinant Toxins
RNA-Sequencing of Snake Venom Glands
Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics
Three-Dimensional Structure Determination of Peptides using Solution Nuclear Magnetic Resonance Spectroscopy
Determining the Structures of the Snake and Spider Toxins by X-Rays
MALDI-TOF Mass Spectrometric Profiling of Spider Venoms
Methods for Evaluation of a Snake Venom Derived Disintegrin in Animal Models of Human Cancer
Cell-Based Adhesion Assays for Isolation of Snake Venom's Integrin Antagonists
Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo
Measurements of Cell Death Induced by Snake and Spider's Venoms and Derived Toxins
Using Toxins in Brain Slice Recordings
High-Throughput Calcium Imaging Screen of Toxins' Function in Dissociated Sensory Neurons
Synthesizing and Expressing Native Ion Channels.
Other Format:
Printed edition:
ISBN:
978-1-4939-9845-6
9781493998456
Access Restriction:
Restricted for use by site license.

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