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Beta-Arrestins : Methods and Protocols / edited by Mark G. H. Scott, Stéphane A. Laporte.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1064-3745 ; 1957.
- Methods in Molecular Biology, 1064-3745 ; 1957
- Language:
- English
- Subjects (All):
- Proteins.
- Cytology.
- Protein Science.
- Cell Biology.
- Receptors.
- Local Subjects:
- Protein Science.
- Cell Biology.
- Receptors.
- Physical Description:
- 1 online resource (XIV, 409 pages) : 79 illustrations, 60 illustrations in color.
- Edition:
- First edition 2019.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana, 2019.
- System Details:
- text file PDF
- Summary:
- This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson's disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.
- Contents:
- A Brief History of the b-Arrestins
- b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling
- Methods to Monitor the Trafficking of β-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET
- Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle
- Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer
- Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET
- Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin
- Methods to Investigate Arrestins in Complex with Phosphodiesterases
- Methods to Characterize Protein Interactions with β-arrestin in cellulo
- Methods to Investigate the β-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling
- Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing
- Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners
- Workflow Description to Dynamically Model β-Arrestin Signaling Networks
- Proteomic Analysis of the β-Arrestin Interactome
- Quantitating Ligand Bias using the Competitive Model of Ligand Activity
- Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins
- Monitoring β-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy
- A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in β-Arrestins
- Probing Arrestin Function using Intramolecular FlAsH-BRET Biosensors
- Methods to Study the Roles of b-Arrestins in Meningococcal Signaling
- Methods to Investigate the Roles for β-Arrestin2 in Allergic Inflammatory Airway Disease
- Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function
- Methods to Investigate β-Arrestin Function in Metabolic Regulation
- Methods to Investigate the Role of b-Arrestin Signaling in Parkinson's Ddisease
- Methods to Investigate β-Arrestin-1/β-Catenin Signaling in Ovarian Cancer Cells.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-4939-9158-7
- 9781493991587
- Access Restriction:
- Restricted for use by site license.
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