Targeting Enzymes for Pharmaceutical Development : Methods and Protocols / edited by Nikolaos E. Labrou.

Format:

Book

Contributor:

Labrou, Nikolaos E., editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 2089.

Methods in Molecular Biology, 1064-3745 ; 2089

Language:

English

Subjects (All):

Pharmaceutical technology.

Enzymology.

Pharmaceutical chemistry.

Pharmaceutical Sciences/Technology.

Medicinal Chemistry.

Local Subjects:

Pharmaceutical Sciences/Technology.

Enzymology.

Medicinal Chemistry.

Physical Description:

1 online resource (XI, 290 pages) : 113 illustrations, 71 illustrations in color.

Edition:

First edition 2020.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2020.

System Details:

text file PDF

Summary:

This volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals. Consisting of three sections, the book delves into recent biocomputing and bioinformatics protocols, state-of-the art modern biophysical, electrophoretic, and chromatographic methods and high-throughput screening approaches, as well as detailed protocols and examples of the inhibition analysis and evaluation of selected enzymes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Targeting Enzymes for Pharmaceutical Development: Methods and Protocols serves as a vital reference for academics and industry professionals working on expanding our understanding of the wide range of important enzyme targets.

Contents:

In Silico Laboratory: Tools for Similarity-Based Drug Discovery

The In Silico Fischer Lock-and-Key Model: The Combined Use of Molecular Descriptors and Docking Poses for the Repurposing of Old Drugs

Determination of Half-Maximal Inhibitory Concentration of an Enzyme Inhibitor

Applications of Differential Scanning Fluorometry and Related Technologies in Characterization of Protein-Ligand Interactions

High-Throughput Differential Scanning Fluorimetry of GFP-Tagged Proteins

Enzyme-Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism

Measuring Small Molecule Binding to Escherichia coli AcrB by Surface Plasmon Resonance

Systematic Screening of Viral Entry Inhibitors Using Surface Plasmon Resonance

Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry

Electrophoretic Mobility Shift Assays with GFP-Tagged Proteins (GFP-EMSA)

On-Line Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors

Enzymatic Bioautographic Methods

High-Throughput Assessment of Metabolism-Induced Toxicity of Compounds on a 384-Pillar Plate

Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors

Ligand Fishing: An Approach for the Discovery Inhibitors from Complex Biological Mixtures

HMG-CoA Reductase as Target for Drug Development

Lipoxygenases as Targets for Drug Development

Cholinesterase as a Target for Drug Development in Alzheimer's Disease.

Other Format:

Printed edition:

ISBN:

978-1-0716-0163-1

9781071601631

Access Restriction:

Restricted for use by site license.