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Multi-Parametric Live Cell Microscopy of 3D Tissue Models / edited by Ruslan I. Dmitriev.

LIBRA Per. v.1 (1967)-v.111 (1979) v.112 (1980)-v.267 (1990), v.269 (1990)-v.330 (1993), v.332 (1993)-v.363 (1995), v.365 (1994)-v.367 (1995), v.369 (1995)-v.370 (1994), v.371B (1995), v.372 (1995)-v.373 (1995), v.375 (1995)-v.401 (1996), v.403 (1996), v.406 (1997), v.408 (1997)-v.482 (2000)-v.483 (2000)
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Holman Biotech Commons R850.A1 A39 v.484 (2001)-v.506 Pt.A (2002), v.506 Pt.B (2002), v.507 (2002)-v.538 (2003), v.539 Pt.A-B (2003), v.540 (2003)-v.554 (2004), v.557 (2006)-v.561 (2005), v.563 (2005)-v.585 (2006),v.587 (2006)-v.600 (2007), v.601 (2007)-v.611 v.612-v.664,v.669-v.681,v.682-v.687,v.689-v.694,v.696-v.698,v.700-v.730,v.732-v.740,v.742-v.751,v.754-v.758,v.760,v.762-v.765,v.767-v.771,v.774-v.780,v.782-v.788,v.790,v.942,v.946,v.954,v.961,v.970,v.984-v.986,v.990-v.992
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Format:
Book
Contributor:
Dmitriev, Ruslan I., editor.
SpringerLink (Online service)
Series:
Biomedical and Life Sciences (Springer-11642)
Advances in experimental medicine and biology 0065-2598 ; 1035.
Advances in Experimental Medicine and Biology, 0065-2598 ; 1035
Language:
English
Subjects (All):
Regenerative medicine.
Tissue engineering.
Microscopy.
Biomedical engineering.
Spectrum analysis.
Regenerative Medicine/Tissue Engineering.
Biological Microscopy.
Biomedical Engineering/Biotechnology.
Biomedical Engineering and Bioengineering.
Spectroscopy and Microscopy.
Local Subjects:
Regenerative Medicine/Tissue Engineering.
Biological Microscopy.
Biomedical Engineering/Biotechnology.
Biomedical Engineering and Bioengineering.
Spectroscopy and Microscopy.
Physical Description:
1 online resource (XII, 172 pages) : 53 illustrations, 39 illustrations in color.
Edition:
First edition 2017.
Contained In:
Springer eBooks
Place of Publication:
Cham : Springer International Publishing : Imprint: Springer, 2017.
System Details:
text file PDF
Summary:
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
Contents:
Current state-of-the-art 3D tissue models and their compatibility with live cell imaging
Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation
Quantitative live cell FLIM imaging in three dimensions
Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview
Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues
Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture
Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2
Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell
Quantitative imaging of Ca2+ by 3D-FLIM in live tissues
Live cell imaging of viscosity in 3D tumour cell models
Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy
Raman imaging microscopy for quantitative analysis of biological samples.
Other Format:
Printed edition:
ISBN:
978-3-319-67358-5
9783319673585
Access Restriction:
Restricted for use by site license.

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