Post-translational modifications that modulate enzyme activity / edited by Benjamin A. Garcia.

Format:

Book

Contributor:

Garcia, Benjamin A., editor.

Series:

Methods in enzymology ; Volume 626.

Methods in enzymology ; Volume 626

Language:

English

Subjects (All):

Post-translational modification.

Physical Description:

1 online resource (642 pages).

Place of Publication:

Cambridge, Massachusetts : Academic Press, [2019]

Summary:

Post-translational Modifications That Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High-throughput phosphoproteome mapping through multiplexed mass spectrometry, Differentiation of D and L epimerization in proteins, Biochemical analysis of protein arginylation, Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale, Genomic and biochemical analysis of RNA post-transcriptional modifications, Isolation and characterization of glycosylated (neuro)peptides, and more.- Provides the authority and expertise of leading contributors from an international board of authors- Presents the latest release in the Methods in Enzymology series- Includes the latest information on Post-translational Modifications that Modulate Enzyme Activity

Contents:

Crosstalk between cellular metabolism and histone acetylation / Sophie Trefely, Mary T. Doan, and Nathaniel W. Snyder

Purification and enzymatic assay of class I histone deacetylase enzymes / Mark K. Adams, Charles A.S. Banks, Sayem Miah, Maxime Killer, and Michael P. Washburn

Multiplexed quantitative phosphoproteomics of cell line and tissue samples / Johannes Kreuzer, Amanda Edwards, and Wilhelm Haas

Differentiation of peptide isomers and epimers by radical-directed dissociation / Tyler R. Lambeth and Ryan R. Julian

Biochemical analysis of protein arginylation / Junling Wang, John R. Yates III, and Anna Kashina

Site-specific determination of lysine acetylation stoichiometries on the proteome-scale / Yue Chen and Yunan Li

RNA modifications and the link to human disease / Amber Yanas and Kathy Fange Liu

Isolation and characterization of glycosylated neuropeptides / Yang Liu, Qinjingwen Cao, and Lingjun Li

Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins / Igor Maksimovic, Devin Ray, Qingfei Zheng, and Yael David

Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomics / Fangxu Sun and Ronghu Wu

Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists / Sourav Chatterjee, Tesia N. Stephenson, Austen L. Michalak, Kamil Godula, and Mia L. Huang

Biochemical and structural analysis of N-terminal acetyltransferases / Leah Gottlieb and Ronen Marmorstein

Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometry / Peng Li, Yuanli Zhen, and Yonghao Yu

Analysis of the topology of ubiquitin chains / Lucia Geis-Asteggiante, Amanda E. Lee, and Catherine Fenselau

Analysis of cardiac troponin proteoforms by top-down mass spectrometry / Timothy N. Tiambeng, Trisha Tucholski, Zhijie Wu, Yanlong Zhu, Stanford D. Mitchell, David S. Roberts, Yutong Jin, and Ying Ge

Assays for tyrosine phosphorylation in human cells / Monica Kruk, Naomi Widstrom, Sampreeti Jena, Nicole L. Wolter, John F. Blankenhorn, Ibrahim Abdalla, Tzu-Yi Yang, and Laurie L. Parker

Metabolomics analysis of lipid metabolizing enzyme activity / Timothy B. Ware, Myungsun Shin, and Ku-Lung Hsu

Simplified high yield TAILS terminomics using a new HPG-ALD 800K-2000 polymer with precipitation / Nestor Solis, Anilkumar Parambath, Srinivas Abbina, Jayachandran Kizhakkedathu, and Christopher M. Overall

Methods for the expression, purification, and crystallization of histone deacetylase 6-inhibitor complexes / Jeremy D. Osko and David W. Christianson

Quantitative analysis of global protein lysine methylation by mass spectrometry / Peder J. Lund, Stephanie M. Lehman, and Benjamin A. Garcia

The roles of S-nitrosylation and S-glutathionylation in Alzheimer's disease / Ryan R. Dyer, Katarena I. Ford, Renã A.S. Robinson

Expression of authentic post-translationally modified proteins in organisms with expanded genetic codes / Kyle Mohler and Jesse Rinehart

Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexes / Nicholas J. Porter and David W. Christianson

Methods for characterizing protein acetylation during viral infection / Laura A. Murray, Ashton N. Combs, Pranav Rekapalli, and Ileana M. Cristea.

Notes:

Description based on print version record.

ISBN:

0-12-818670-4

0-12-818669-0