In Vitro Fertilization : A Textbook of Current and Emerging Methods and Devices / edited by Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal.

Format:

Book

Contributor:

Nagy, Zsolt Peter, editor.

Varghese, Alex C., editor.

Agarwal, Ashok (Physician), editor.

SpringerLink (Online service)

Series:

Medicine (Springer-11650)

Language:

English

Subjects (All):

Reproductive health.

Endocrinology.

Reproductive Medicine.

Local Subjects:

Reproductive Medicine.

Endocrinology.

Physical Description:

1 online resource (XXII, 962 pages) : 213 illustrations, 198 illustrations in color

Edition:

Second edition 2019.

Contained In:

Springer eBooks

Place of Publication:

Cham : Springer International Publishing : Imprint: Springer, 2019.

System Details:

text file PDF

Summary:

Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.

Contents:

Journey of Human Gametes In Vitro

1978 to 2018

Setting up an ART unit - Planning, Design and Construction

Building the Laboratory

Air Quality Management

Organization And Workflow In A Fertility Clinic

Risk and Safety in the IVF Clinic

Control of variables

Daily, Weekly, and Regular Preparations for the IVF Laboratory

Essential Instruments and Disposable Supplies for an IVF Laboratory

Co2 and Low-O2 Incubators

What to consider when selecting a LAF, Class II Cabinet or Isolette for your ART program?

Culture Media in IVF: Decisions for the Laboratory

Assessment Of Oocyte Quality

Oocyte Denuding

Interrupted or Uninterrupted Culture to the Blastocyst Stage in a Single Medium

Sequential Media for Human Blastocyst Culture

In Vitro Maturation of Human Oocytes

Design and Development of Simplified, Low Cost Technologies for Clinical IVF: Applications in High and Low Resource Settings

In vitro culture of oocytes and embryos with micro-vibration: "naturalization" of routine IVF procedure

Microfluidics for Gamete Manipulation and Embryo Culture

Time-Lapse Microscopy for Embryo Culture and Selection

Sperm Assessment: Traditional Approaches and Their Indicative Value

Sperm Assessment: Novel Approaches and Their Indicative Value

Assessment of Sperm Chromatin Damage by TUNEL Method Using Bench Top Flow Cytometer

Sperm processing in ART

PESA/MESA/TESA/TESE Sperm Processing

Processing Sperm Samples in HIV-Positive Patients

An Emerging Medical Device: Electrophoretic Sperm Separation

Magnetic Activated Cell Sorting of Human Spermatozoa

Intrauterine Insemination

Conventional IVF Insemination

Intra-Cytoplasmic Sperm Injection

ntracytoplasmic Morphologically Selected Sperm Injection

Intracytoplasmic Morphologically Selected Sperm Injection

Oocyte Activation Deficiency and Advances to Overcome

Hydraulic Manipulators for ICSI

Research Instruments Micromanipulation Systems

Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators.-Oocyte Treatment and Preparation for Microinjection

Piezo-ICSI

Traditional Embryo Morphology Evaluation: From the Zygote to the Blastocyst Stage

Predicting Embryo Implantation Potential Using Video Monitoring By the Embryoscope Time-Lapse System

Predicting Embryo Developmental Potential and Viability Using Automated Time-Lapse Analysis

Proteomics and Metabolomics

Amino Acid Turnover as a Biomarker of Embryo Viability

Microfluidics and Microanalytics to Facilitate Quantitative Assessment of Human Embryo Physiology

Assisted Hatching of Human Embryos for Successful Implantation

Polar Body Biopsy

Cleavage-Stage Embryo Biopsy

Current and Novel Methods for Chromosome Testing.-Embryo Biopsy for PGD: Current Perspective

Sperm Cryopreservation

Technologies of Cryoprotectant-free Vitrification of Human Spermatozoa: Asepticity as Criterionn of Effectiveness

Slow Freezing of Oocytes

Vitrification: Methods Contributing to Successful Cyopreservation Outcomes

Oocyte Vitrification and Current Clinical Applications

Slow Freezing of Embryos

Vitrification of Embryos

Ovarian Tissue Cryopreservation

Establishing and Managing Donor Oocyte Banking

Single Embryo Transfer

Embryo Transfer: Techniques and Trouble shooting

G-CSF and GM-CSF: Clinical applications in reproductive medicine

The Freeze All Cycle: A New Paradigm Shift in ART

IVF Data Management: From Clipboards to Smart Apps

Regulation of the ART Laboratory

Legislation in the UK

Regulation, Licensing, and Accreditation of the ART Laboratory in Europe

Regulation, Licensing, and Accreditation of the ART Laboratory in Brazil

Regulation, Licensing and Accreditation of ART Laboratories in Australia

Trouble Shooting In Ivf Laboratories

Benchmarking and Decision Making in the IVF Laboratory

Automation, Artificial Intelligence and innovations in the future of IVF

Assisted reproductive technologies to prevent transmission of mitochondrial DNA disease

Nuclear and Cytoplasmic Transfer: Human Applications and Concerns

Embryo culture and phenotype of the offspring

Traceability in ART

The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development

Cytoskeletal architecture of human oocytes with a focus on centrosomes and their significant role in fertilization

Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence

Livestock Production via Micromanipulation. .

Other Format:

Printed edition:

ISBN:

978-3-319-43011-9

9783319430119

Access Restriction:

Restricted for use by site license.