3D cell-based biosensors in drug discovery programs : microtissue engineering for high throughput screening / William S. Kisaalita.

Format:

Book

Author/Creator:

Kisaalita, William Ssempa, 1953-

Language:

English

Subjects (All):

Pharmaceutical biotechnology.

Biosensors.

High throughput screening (Drug development).

Physical Description:

1 online resource (392 p.)

Edition:

1st ed.

Place of Publication:

Boca Raton, [FL] : CRC Press, 2010.

Language Note:

English

Summary:

This book is based upon cutting-edge research conducted in the authors lab (Cellular Bioengineering), which over the past decade has developed a number of sophisticated techniques to facilitate use of 3D cell based assays or biosensors. This book uses data from peer-reviewed publications to conclusively justify use of 3D cell cultures in cell-based biosensors (assays) for (HTS). The majority of assays performed in accelerated drug discovery processes are biochemical in nature, but there is a growing demand for live cell-based assays. Unlike biochemical ones, cellular assays are functional approximations of in vivo biological conditions and can provide more biologically relevant information--Provided by publisher.

Contents:

Front Cover; Dedication; Contents; Preface; Author; Part I. Introduction; Chapter 1. Biosensors and Bioassays; Chapter 2. Target-Driven Drug Discovery; Part II. 3D versus 2D Cultures; Chapter 3. Comparative Transcriptional Profiling and Proteomics; Chapter 4. Comparative Structure and Function; Part III. Emerging Design Principles; Chapter 5. Chemical Microenvironmental Factors; Chapter 6. Spatial and Temporal Microenvironmental Factors; Chapter 7. Material Physical Property and Force Microenvironmental Factors; Chapter 8. Proteomics as a Promising Tool in the Search for 3D Biomarkers

Chapter 9. Readout Present and Near FutureChapter 10. Ready-to-Use Commercial 3D Plates; Part IV. Technology Deployment Challenges and Opportunities; Chapter 11. Challenges to Adopting 3D Cultures in HTS Programs; Chapter 12. Cases for 3D Cultures in Drug Discovery; Chapter 13. Ideal Case Study Design; Appendix A: Patents for 3D Scaffolds; Appendix B: Current Drug Targets; Appendix C: Popular Cell Lines in Drug Discovery; Appendix D: Stem Cells in Drug Discovery; Back Cover

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on metadata supplied by the publisher and other sources.

ISBN:

1-4398-5905-1

0-429-14677-9

1-4200-7350-8

9780429146770

OCLC:

660536815